A cutting-edge collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion and tried, as well as true step-by-step instructions.
This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them...a very comprehensive methods book that will be a laboratory standard for years to come. - Microbiology Today ...this book constitutes an excellent reference text ...this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses. - FEBS Letters
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