Gerard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded to the isolation, subcellular fractionation, and transfection of low density adipocytes as well as metabolic aspects of nutrient uptake and assays of nutrient and ion fluxes. Innovative and highly practical, "Adipose Tissue Protocols" offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective research on adipose tissue from the nutritional to the physiological and the molecular levels.
Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity, Louis Casteilla, Luc Penicaud, Beatrice Cousin, and Denis Calise. Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue, Saverio Cinti, M. Cristina Zingaretti, Raffaella Cancello, Enzo Ceresi, and Plinio Ferrara. Confocal Microscopy of Adipocytes, Daniela Malide. Cellularity Measurements, Mario DiGirolamo and Jacqueline B. Fine. Subcellular Fractionation of Adipocytes and 3T3-L1 Cells, Hans-Georg Joost and Annette Schurmann. Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies, Hubert Vidal. Glycogen Synthase Activity in Adipose Tissue: Methods for Freeze-Clamping and Assay, Heidi K. Ortmeyer. Assays of Lipolytic Enzymes, Cecilia Holm, Gunilla Olivecrona, and Malin Ottosson. Assays of Lipogenic Enzymes, Raymond Bazin and Pascal Ferre. Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements, Christian Carpene. Transfection of Adipocytes and Preparation of Nuclear Extracts, Isabelle Dugail. Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes, Nada A. Abumrad. Assays of Glucose Entry, Glucose Transporter Amount, and Translocation, Jean-Francois Tanti, Mireille Cormont, Thierry Gremeaux, and Yannick Le Marchand-Brustel. Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes, Eva Degerman, Svante Resjo, Tova Rahn Landstrom, and Vincent Manganiello. Measurements of Glucose Conversion to its Metabolites, Mario DiGirolamo. Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes, Jan Nedergaard. Culture of Adipose Tissue and Isolated Adipocytes, Susan K. Fried and Naima Moustaid-Moussa. Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines, Barbara Cannon and Jan Nedergaard. Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue, Raymond Negrel and Christian Dani. Cultures of Human Adipose Precursor Cells, Hans Hauner, Thomas Skurk, and Martin Wabitsch. Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes, Marie-Christine Alessi, Jerome Aubert, and Raymond Negrel. Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences, Keith N. Frayn and Simon W. Coppack. Measurement of Adipose Tissue Blood Flow, Jens Bulow. Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue, Barbara Cannon and Jan Nedergaard. Measurements of White Adipose Tissue Metabolism by the Microdialysis Technique, Pierre Barbe, Christian Darimont, Perla Saint-Marc, and Jean Galitzky. Immunoassay Measurements of Leptin, Martin Bidlingmaier and Matthias Tschoep. Index.
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