Agrobacterium Protocols

Handbook / Manual Out of Print

Series: Methods in Molecular Biology Volume: 44

Edited By: KMA Gartland and M Davey

470 pages, Illus

Publisher: Humana Press

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ISBN: 9780896033023 Hardback Dec 1995 Out of Print #43276

About this book Contents

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About this book

Examines protocols for the expression of agrobacterium genes in higher plants.

Growth and Storage of Agrobacterium. Simple Cultural Tests for Identification of Agrobacterium Biovars. Agrobacterium Virulence. Agrobacterium tumefaciens Chemotaxis Protocols. Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens. Binary Ti Plasmid Vectors. Leaf Disk Transformation. Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.). Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.). Peanut Transformation. Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants. Agrobacterium-Mediated Transformation of Potato Genotypes. Agrobacterium-Mediated Transformation of Soft Fruit Rubus Ribes and Fragaria. High-Frequency and Efficient Agrobacterium-Mediated Transformation of Arabidopsis thaliana Ecotypes C24 and Landsberg erecta Using Agrobacterium tumefaciens. Transformation Protocols for Broadleaved Trees. Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids: The Genus Lycopersicon as a Model System. Histochemical GUS Analysis. Fluorometric GUS Analysis for Transformed Plant Material. The Detection of Neomycin Phosphotransferase Activity in Plant Extracts. The Plant Oncogenes rolA B and C from Agrobacterium rhizogenes: Effects on Morphology Development and Hormone Metabolism. Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells. IAA Analysis in Transgenic Plants. Quantifying Phytohormones in Transformed Plants. Manipulating Photosynthesis in Transgenic Plants. Gene Activation by T-DNA Tagging. Assessing Cadmium Partitioning in Transgenic Plants. Overepression of Chloroplastic Cu/Zn Superoxide Dimutase in Plants. Agroinfection. T-DNA Transfer to Maize Plants. Use of Cosmid Libraries in Plant Transformations. Regulation of Agrobacterium Gene Manipulation. Transgenic Oilseed Rape: How to Assess the Risk of Outcrossing to Wild Relatives. Electroporation Protocols for Agrobacterium. Index.