Antibody Techniques

About this book

The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Key features include: detailed, easy-to-follow, step-by-step protocols; convenient, easy-to-use format; extensive practical information; essential background information, and helpful hints.

Contents

I.M. Zitron, Antibody Molecules and the Immune Response. D.P. Snider, Adjuvant-Free Polyclonal Antibody Response Manipulated by Antibody-Mediated Antigen Targeting. C. Barrett, Hybridomas and Monoclonal Antibodies. J.W. Larrick and R.F. Balint, PCR Amplification and Antibody Genes. P.C. Allen, Laboratory Animal Care and Use. A.C. Allison and N.E. Byars, Adjuvants. C. Glad and I. Nilsson, Large-Scale Production of Monoclonal Antibodies. D.P. Boyle and D.W. Metzger, Antibody-Binding Bacterial Proteins as Immunoreagents. B.-K. Choe, Conjugating Antibodies to Ligands, Enzymes, and Metallic Particles. E. Lauritzen, H. Flyge, and A. Holm, Dot Immunobinding (DIB), Enzyme Linked Immunosorbent Assay (ELISA) and Radioimmunoassay (RIA) for Detection of Peptide Antigens and Specific Antibodies. B.-K. Choe and Y. Cho, Immunostaining Cells and Tissues. E.P. Lillehoj, Protein Immunoblotting. H.S. Lillehoj and A.G. Martin, Flow Cytometry and Fluoresence-Activated Cell Sorting. W.J. Patrie, Screening Expression Libraries with Antibodies. J.P. Tharakan, Immunoaffinity Purification.

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