Antibody Phage Display Methods and Protocols

About this book

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today's powerful phage display technology.

Contents

Overview of Antibody Phage Display Technology and its Applications Hennie R. Hoogenboom Standard Protocols for the Construction of Fab Libraries Michelle A. Clark Standard Protocols for the Construction of scFv Libraries Simon Lennard Broadening the Impact of Antibody Phage Display Technology: Amplification of Immunoglobulin Sequences from Species Other than Humans or Mice Philippa M. O'Brien and Robert Aitken Construction of Large Naive Fab Libraries Hans J. W. de Haard Construction of Polyclonal Antibody Libraries Using Phage Display Jacqueline Sharon, Seshi R. Sompuram, Chiou-Ying Yang, Brent R. Williams and Stefanie Sarantopoulos Antigen-Driven Stimulation of B Lymphocytes In Vitro Zhiwei Hu The Recovery of Immunoglobulin Sequences from Single Human B Cells by Clonal Expansion Ruud M. T. de Wildt and Rene M. A. Hoet Panning of Antibody Phage Display Libraries: Standard Protocols David W. J. Coomber Selection of Antibodies Against Biotinylated Antigens Patrick Chames, Hennie R. Hoogenboom and Paula Henderikx Isolation of Anti-Hapten Specific Antibody Fragments from Combinatorial Libraries Keith A. Charlton and Andrew J. Porter Blocking Immunodominant Epitopes by Competitive Deselection Roberto Burioni Rescue of a Broader Range of Antibodies Using an Epitope-Masking Strategy Henrik J. Ditzel Screening of Phage-Expressed Antibody Libraries by Capture Lift Jeffry D. Watkins Antibody-Guided Selection Using Capture Sandwich ELISA Kunihiko Itoh and Toshio Suzuki Proximity-Guided (ProxiMol) Antibody Selection Jane Osbourn Isolation of Human mAbs Using Guided Selection With Mouse mAbs Mariangela Figini and Silvana Canevari Selecting Antibody to Cell Surface Antigens Using Magnetic Sorting Techniques Don L. Siegel Isolation of Human Tumor-Associated Cell Surface Antigen binding scFv Fragments E. Jacintha Noronha, Xinhui Wang and Soldano Ferrone Subtractive Isolation of Single Chain Antibodies Using Tissue Fragments Katarina Radosevic and Willem van Ewijk Selection of Antibodies Based on Antibody Kinetic Binding Properties Ann-Christin Malmborg, Nina Nilsson and Mats Ohlin Selection of Functional Antibodies on the Basis of Valency Manuela Zaccolo Two-Step Strategy for Alteration of Immunoglobulin Specificity by in vitro Mutagenesis Yoshitaka Iba, Chie Miyazaki and Yoshikazu Kurosawa Targeting Random Mutations to Hotspots in Antibody Variable Domains for Affinity Improvement Partha S. Chowdhury Use of Error-Prone PCR for the Modification of Single-Chain Fv Fragments Pierre Martineau Use of E. coli Mutator Cells to Mature Antibodies Robert Irving, Gregory Coia, Anna Raicevic and Peter J. Hudson Chain Shuffling to Modify the Properties of Recombinant Immunoglobulins Johan Lantto, Pernilla Jirholt, Yvelise Barrios and Mats Ohlin Generation of Bispecific and Tandem Diabodies Sergey M. Kipriyanov High Level Periplasmic Expression and Purification of scFv Fragments Sergey M. Kipriyanov Periplasmic Expression and Purification of Recombinant Fab Fragments Robert L. Raffai Expression of Antibody Fragments in Pichia pastoris Philipp Holliger Expression of VHH Antibody Fragments in Saccharomyces Cerevisiae J. Marcel van der Vaart Intrabodies: Targeting scFv Expression to Eukaryotic Intracellular Compartments Pascale A. Cohen Expression of scFvs and scFv-Fusion Proteins in Eukaryotic Cells Michelle de Graaf, Ida van der Muelen-Muileman and Hidde J. Haisma Expression of Antibody Fabs Fragments and Whole Ig in Mammalian Cells Pietro P. Sanna

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