Apoptosis: Methods and Protocols

About this book

The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research.

In "Apoptosis: Methods and Protocols", expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, "Apoptosis: Methods and Protocols" constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death.

Contents

I. Detection of Apoptosis
1. Analyzing Caspase Activation and Caspase Activity in Apoptotic Cells
Sharad Kumar and Loretta Dorstyn
2. Flow Cytometry-Based Apoptosis Detection
Donald Wlodkowicz, Joanna Skommer, and Zbigniew Darzynkievicz
3. Live to Dead Cell Imaging
Stephen W.G. Tait, Lisa Bouchier-Hayes, Andrew Oberst, Samuel Connell, and Douglas R. Green
4. Detection of Apoptosis in Tissue Sections
Eva Csizmadia and Vilmos Csizmadia
5. Detection of Apoptosis in Cell-Free Systems
Dhyan Chandra and Dean G. Tang

II. Detection of Non-Apoptotic Cell Death
6. Methods to Analyze Cellular Necroptosis
Benchun Miao and Alexei Degterev
7. Detection of Cell Death by Autophagy
Narasimman Gurusamy and Dipak K. Das

III. Modifications of Apoptotic Proteins during Apoptosis
8. Methods to Analyze Transglutamination of Proteins Involved in Apoptosis
Zoltan Nemes and Laszlo Fesus
9. Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis
Neelam Azad, Anand Krishnan V. Iyer, and Yon Rojanasakul
10. Application of in vivo EPR for Tissue pO2 and Redox Measurements
Nadeem Khan and Dipak K. Das

IV. Analysis of the Function of Major Regulators of Apoptosis/Cell Survival
11. Assays to Measure p53-dependent and Independent Apoptosis
Darren . Phillips, Sean P. Garrison, John R. Jeffers, and Gerard P. Zambetti
12. Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis
Ramon Roset and Gabriel Gil-Gomez
13. Fluorimetric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis
Soumya Sinha Roy and Gyorgy Hajnoczky
14. Regulation of Apoptosis by the Unfolded Protein Response
Andrew Fribley, Kezhong Zhang, and Randal Kaufman
15. Detection of Uncoupling Protein-2 (UCP-2) as a Mitochondrial Modulator of Apoptosis
Zoltan Derdak, Tamako A. Garcia and Gyorgy Baffy
16. A Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis
Riccardo Spizzo and George Calin
17. Assessment of Apoptotic Cell Phagocytosis by Macrophages
Kathleen A. McPhilips and Lars-Peter Erwig

V. Analysis of Apoptosis in Different Organs
18. Detection of Apoptosis in Mammalian Development
Lin Lin, Carlos Penalosa, Yixia Ye, Richard A. Lockshin, and Zahra Zakeri
19. Detection of Apoptosis in the Central Nervous System
Youngsoo Lee and Peter J. McKinnon
20. Genetic Mapping of Anti-apoptosis Pathways in Myeloid Progenitor Cells
Dan Liu and Zhou Songyang
21. Analysis of Apoptosis in Isolated Primary Cardiac Myocytes
Adel Mandl, Ambrus Toth, and Peter Erhardt
22. Cell Death in Myoblasts and Muscles
Lawrence M. Schwartz, Zhengliang Gao, Christine Brown, Sangram S. Parelkar, and Honor Glenn

VI. Analysis of Apoptosis in Model Organisms
23. Reliable Method for Detection of Programmed Cell Death in Yeasts
Xinchen Teng and J. Marie Hardwick
24. Detection of Cell Death in Drosophila
Kimberly McCall, Jeanne S. Peterson, and Tracy L. Pritchett
25. Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans
Nan Lu, Xiaomeng Yu, Xiangwei He, and Zheng Zhou
26. Detection of Herpes Simplex Virus Dependent Apoptosis
Christopher R. Cotter and John A. Blaho

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