Basic Cell Culture Protocols

About this book

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, Second Edition offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Contents

Basic Cell Culture Jeffrey W. Pollard. Establishment Maintenance and Cloning of Human Dermal Fibroblasts Gareth E. Jones and Clare J. Wise. Aging of Cultured Human Skin Fibroblasts Calvin B. Harley and Steven W. Sherwood. Ex Vivo Maintenance of Differentiated Mammalian Cells Lola M. Reid and Thomas L. Luntz. Scale-Up of Suspension and Anchorage-Dependent Animal Cells Bryan Griffiths and Denis Looby. Hollow-Fiber Cell Culture John M. Davis and Julian A. J. Hanak. Separation and Maintenance of Primary T-and B-Lymphocytes Derek Kinchington and Tony Ng. Human Pilosebaceous Culture Terence Kealey Michael Philpott and Robert Guy. Keratinocyte Culture Yvonne Barlow and Richard J. Pye. Tissue Culture of Skeletal Muscle Terry A. Partridge. Isolation of Rat Liver Hepatocytes David S. Neufeld. Primary Kidney Cells Mary Taub. Human Thyroid Epithelial Cells D. W. Williams and David Wynford-Thomas. Derivation and Maintenance of Embryonic Stem Cell Cultures Elizabeth J. Robertson. Culture of Cells From Human Tumors of the Nervous System on an Extracellular Matrix Derived From Bovine Corneal Endothelial Cells Manfred Westphal Alf Giese Hildegard Meissner Dorothea Zirkel. Culturing Primitive Hemopoietic Cells Elaine Spooncer and T. Michael Dexter. Collagen Gel Culture of the Human Hematopoietic Progenitors CFU-GM CFU-E and BFU-E Annie Allegraud Ir'ne Dobo and Vincent Praloran. Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cell Line AC 6.21 Cheryl A. Whitlock and Christa E. Muller-Sieburg. Human Long-Term Bone Marrow Culture Brian R. Clark Catriona Jamieson and Armand Keating. In Vitro Clonal Culture of Human Hematopoietic Progenitor Cells Brian R. Clark Catriona Jamieson and Armand Keating. High Proliferative Potential Colony Forming Cells Ivan Bertoncello and Anthony B. Kriegler. Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors Malcolm Strath Elaine J. Clutterbuck and Colin J. Sanderson. Flow Sorting for Isolating CFU-E Suzanne M. Watt and John M. Davis. Murine Bone Marrow-Derived Macrophages E. Richard Stanley. Routine Testing of Cell Cultures and Their Products for Mycoplasma Contamination Alison Stacey and Alan Doyle. Fluorescent In Situ Hybridization (FISH) for DNA Probes in the Interphase and Metaphases Stages of the Cell Cycle Linda A. Cannizzaro and Guangping Shi. Cytogenetic Techniques for Human Leukemias John Swansbury. Visualization of Cell Replication Using Antibody to Proliferating Cell Nuclear Antigen James Hyde-Dunn and Gareth E. Jones. Proliferation of Murine Myoblasts as Measured by BrdUrd Incorporation Bina Shah James Hyde-Dunn and Gareth E. Jones. Analysis of Cell Proliferation Using the Bromodeoxyuridine/Hoechst-Ethidium Bromide Method M. G. Ormerod. mRNA In Situ Hybridization to In Vitro Cultured Cells Anton K. Raap Frans M. van de Rijke and Roeland W. Dirks. Transmission and Scanning Electron Microscope Preparation of Whole Cultured Cells Josef Neum?ller. Adhesion Molecules on Isolated and Cultured Microvascular Endothelial Cells Demonstrated by Immunofluorescence and Immune Electron Microscopy Josef Neum?ller and Johannes Menzel. Production of Heterologous Proteins Using the Baculovirus/Insect Expression System Caroline M. Griffiths and Martin J. Page. Transfection and Transformation of Human Thyroid Epithelial Cells Nicholas Robert Lemoine and David Wynford-Thomas. Analyzing Chemotaxis Using the Dunn Direct-Viewing Chamber Daniel Zicha Graham Dunn and Gareth Jones. Computer- Assisted Analysis of Single Cell Behavior Michael Cammer Jeffrey Wyckoff and Jeffrey E. Segall. Appendix: Cell Culture Media Jeffrey W. Pollard. Index.

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