Basic Plant Pathology Methods

About this book

The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations.T he Second Edition includes chapters on the following:oSterilization of culture apparatus and culture mediaoCulture of pathogens with detailed techniques for 61 fungi and selected bacteriaoLong-term storage of plant pathogensoDetection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogensoEstablishment of disease and testing for disease resistanceoWork with soil microorganismsoFungicide evaluationoBiological controloBright-field microscopy

Contents

Sterilization of Apparatus and Culture MediaHeat SterilizationGas SterilizationFiltrationCulture of PathogensFactors Affecting the Cultivation and Sporulation of Pathogenic FungiSome Special Methods of Inducing Sporulation in FungiCultivation of Pathogens for Soil InfestationOther Sources of InoculumCultivation and Sporulation of Some PathogensLong-Term Storage of Plant PathogensPeriodic TransferMineral OilDryingStorage in WaterStorage in Silica GelStorage in SoilFreezingPreservation in Liquid NitrogenLyophilization and Vacuum DryingDetection and Estimation of InoculumSoilborne PathogensDetection and Estimation of Inoculum in the AirDetection of Pathogens Associated with SeedsEstablishment of Disease and Testing for ResistanceFactors Influencing Disease EstablishmentRoot InoculationStem InoculationLeaf InoculationInoculation of Cereal InflorescencesSeed InoculationInoculation with BacteriaInoculation of Detached Plant PartsField Inoculation MethodsSome Special HintsSoil MicroorganismsIsolation and Enumeration of Soil OrganismsObservation of Soil Microorganisms in SituPhysiological Processes as an Estimate of Biological Activity in SoilSpore Germination in Soil and Soil FungistasisSurvival of Plant Pathogens in SoilMycelial Growth in SoilCollection of Root and Seed ExudatesArtificial Rhizosphere or SpermosphereFungicide EvaluationSpore Germination TestTests Based on Growth on Organisms In VitroTransfer-Bioassay TechniquesLaboratory Testing for Soil FungicidesGreenhouse Testing of Foliar FungicidesGreenhouse Testing of Fungicides for Control of Soilborne PathogensPlanning Field ExperimentsSeed Treatment with ChemicalsBiological ControlCollection and Testing of Soil Samples for AntagonistsIsolation of Antibiotic-Producing OrganismsIsolation and Testing of Lytic OrganismsIsolation and Testing of Mycoparasitic OrganismsSpore Perforating AmoebaeIsolation of Antagonists from LeavesTesting Antagonists for Biological ControlBright-Field Microscopy TechniquesMounting and Staining of Fungi for Microscopic ExaminationStaining of Fungal NucelliMounting Spores for Viewing from Different PositionsImmobilization of Fungus Spores in Water Mounts for Microscopic ExaminationStaining of BacteriaCements for Sealing MountsSlide Culture Techniques and Preparation of Permanent MountsPreparing Agar-Colony Mounts and Dry Reference CulturesExamination of Host-Parasite Relationship Using Bright-Field MicroscopyAppendicesCulture Media and Their FormulasPlant Nutrient SolutionsSolutions to Maintain Constant Humidity in a Closed AtmosphereDrying AgentspH Indicator DyesApproximate Figures for Concentrated Acids and BasesPreservation of Museum SpecimensCollection, Preparation, and Mailing of Cultures and SpecimensGlassware Cleaning SolutionsIndex

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