Expert academic and pharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding.
Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis Charles A. Lucy, Nicole E. Baryla, and Ken K.-C. Yeung Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillary Electrophoresis With Laser-Induced Fluorescence Detection Hossein Ahmadzadeh, Norman J. Dovichi, and Sergey Krylov On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection Hossein Ahmadzadeh and Sergey Krylov Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications John Sowell, Jozef Salon, Lucjan Strekowski, and Gabor Patonay Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes Vadim Klyushnichenko Capillary Electrophoresis of Proteins in a Quality Control Environment David L. Good, Stacey Cummins-Bitz, Raeann M. Fields, and Brian K. Nunnally Analysis of Neutral N-Linked Oligosaccharides From Antibodies Using Free-Solution Capillary Electrophoresis in Bare Fused-Silica Capillaries Jeffrey S. Patrick, Brenda P. Rener, Gregory S. Clanton, and Avinash L. Lagu Affinity Capillary Electrophoresis to Examine Receptor Ligand Interactions Maryam Azad, John Kaddis, Valerie Villareal, Lili Hernandez, Catherine Silverio, and Frank A. Gomez Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis Hee Seung Kim, John Austin, and David S. Hage Using Charge Ladders and Capillary Electrophoresis to Measure the Charge, Size, and Electrostatic Interactions of Proteins Upma Sharma and Jeffrey D. Carbeck Frontal Analysis Continuous Capillary Electrophoresis for Protein Polyelectrolyte Binding Studies Emek Seyrek, Toshiaki Hattori, and Paul L. Dubin Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection Jiaqi Wu, Xing-Zheng Wu, Tiemin Huang, and Janusz Pawliszyn Capillary Electrophoresis Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins Mehdi Moini Capillary Isoelectric Focusing Mass Spectrometry of Proteins and Protein Complexes Suzana Martinovic, Ljiljana Pasa-Tolic, and Richard D. Smith Integrated System for Rapid Proteomics Analyses Using Microfluidic Devices Coupled to Nanoelectrospray Mass Spectrometry Jianjun Li, Tammy-Lynn Tremblay, Jed Harrison, and Pierre Thibault Index
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