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As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular BiologyT series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
Chapter 1: Introduction: Cell-based Assays for High-throughput ScreeningW. Frank An and Nicola J. TollidayChapter 2: High-throughput Screening of Model BacteriaSoumaya Zlitni, Jan E. Blanchard, and Eric D. BrownChapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial CellsMichael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas MayerChapter 4: Large-scale Small-molecule Screen Using Zebrafish EmbryosCharles C. HongChapter 5: Whole-animal High-throughput Screens: The C. elegans ModelEyleen J. O'Rourke, Annie L. Conery, and Terence I. MoyChapter 6: Whole-organism Screening: PlantsApril Agee and David CarterChapter 7: Fluorescence-based AssaysW. Frank AnChapter 8: Reporter-gene AssaysAndy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. WongChapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration AssayAruna D. Balgi and Michel RobergeChapter 10: Assay for Isolation of Inhibitors of Her2-kinase ExpressionGabriela Chiosis and Adam B. KeetonChapter 11: ImageJ: A PrimerKatherine Luby-PhelpsChapter 12: High-content Screening: Flow Cytometry AnalysisBruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. SklarChapter 13: High-throughput Real-time PCR for Detection of Gene-expression LevelsBridget K. Wagner and Zoltan AranyChapter 14: Interpretation of Uniform-well ReadoutsSerene JosiahChapter 15: Extracting Rich Information from ImagesAnne E. Carpenter
From the reviews: "'Cell-based assays for high-throughput screening' is a highly practical resource, written by experts in each of the techniques ! . very useful from a practical point of view, and anyone looking to find a new assay system will find a wealth of ideas here. ! aimed at molecular biologists, many other scientists - such as natural product chemists looking for new lead molecules - should find it equally useful." (Elizabeth Williamson, Phytotherapy Research, September, 2009)
