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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Combinatorial Peptide Library Protocols

Handbook / Manual
Series: Methods in Molecular Biology Volume: 87
Edited By: Shmuel Cobilly
320 pages
Publisher: Humana Press
Combinatorial Peptide Library Protocols
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  • Combinatorial Peptide Library Protocols ISBN: 9780896033924 Hardback Dec 1997 Not in stock: Usually dispatched within 1-2 weeks
    £89.99
    #99003
Price: £89.99
About this book Contents Customer reviews Related titles

About this book

This is a valuable collection of new and unique techniques for the construction and use of peptide libraries. These powerful methods include protocols for the chemical synthesis of peptide libraries, for constructing peptide libraries that are displayed on the surface of filamentous phage or bacteria, and for the rapid screening of these libraries for molecules with biospecific properties. Additional methods permit identifying specific enzyme substrates, investigating the recognition spectra of various binding proteins, epitope mapping, and identifying mimotopes. State-of-the-art techniques, combined with clear step-by-step instructions, make this book an essential tool in the selection of peptides suitable for drug development.

Contents

Synthesis of a One-Bead One-Compound Combinatorial Peptide Library Kit S. Lam and Michal Lebl. Enzyme-Linked Colorimetric Screening of a One-Bead One-Compound Combinatorial Library Kit S. Lam. Synthesis and Screening of Positional Scanning Combinatorial Libraries Colette T. Dooley and Richard A. Houghten. Synthesis and Screening of Peptide Libraries on Continuous Cellulose Membrane Supports Achim Kramer and Jens Schneider- Mergener. Peralkylation: Libraries from Libraries: Chemical Transformation of Synthetic Combinatorial Libraries John M. Ostresh Barbara Drner and Richard A. Houghten . Introduction to Combinatorial Solid-Phase Assays for Enzyme Activity and Inhibition Morten Meldal. Preparation of Biocompatible Resins for Library Syntheses Morten Meldal. Intramolecular Fluorescence-Quenched Substrate Libraries Morten Meldal. The Solid-Phase Enzyme Inhibitor Library Assay Morten Meldal. Determination of Peptide Substrate Motifs for Protein Kinases Using a One-Bead One Compound Combinatorial Library Approach Kit S. Lam. The Use of Peptide Library for the Determination of Kinase Peptide Substrates Zhou Songyang and Lewis C. Cantley. Analysis of Protein Kinase Substrate Specificity by the Use of Peptide Libraries on Cellulose Paper (SPOT-Method) Werner J. Tegge and Ronald Frank. Generation of Multiuse Peptide Libraries for Functional Screenings Channa K. Jayawickreme Shiranthi P. Jayawickreme and Michael R. Lerner. Functional Screening of Multiuse Peptide Libraries Using Melanophore Bioassay Channa K. Jayawickreme Shiranthi P. Jayawickreme and Michael R. Lerner. The Basic Structure of Filamentous Phage and Its Use in the Display of Combinatorial Peptide Libraries Shmuel Cabilly. Construction and Use of a 20-mer Phage Display Epitope LibraryBaruch Stern and Jonathan M. Gershoni. Construction of Disulfide-Constrained Random Peptide Libraries Displayed on Phage Coat Protein VIII Alessandra Luzzago and Franco Felici . Conformational Mimicry Through Random Constraints Plus Affinity Selection Guangming Zhong. The Use of Combinatorial Libraries to Identify Ligands That Interact with Surface Receptors in Living Cells Shmuel Cabilly Judith Heldman Eliahu Heldman and Ephraim Katchalski-Katzir. Screening Phage Display Peptide Libraries on Nitrocellulose Membranes Shmuel Cabilly Judith Heldman and Ephraim Katchalski-Katzir. Identification of Disease-Specific Epitopes Atonella Folgori Alessandra Luzzago Paolo Monaci Alfredo Nicosia Riccardo Cortese and Franco Felici. Identification of Peptide Ligands for the Antigen Binding Receptor Expressed on Human B-Cell Lymphomas Markus F. Renschler William J. Dower and Ronald Levy. Major Histocompatibility Complex Allele-Specific Peptide Libraries and Identification of T-Cell Mimotopes Marc A. Gavin and Michael J. Bevan. Phage Display of Peptide Libraries on Protein Scaffolds Henry B. Lowman. Displaying Libraries on Conformationally Constrained Peptides on the Surface of Escherichia coli as Flagellin Fusions Zhijian Lu Brian C. Tripp and John M. McCoy. Combinatorial Peptide Library as an Immunogen J. Estaquier J.-C. Ameisen C. Auriault C. Boutillon H. Gras-Masse and A. Tartar. Index.

Customer Reviews

Handbook / Manual
Series: Methods in Molecular Biology Volume: 87
Edited By: Shmuel Cobilly
320 pages
Publisher: Humana Press
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