Contents include: biotechnology in conservation of genetic resources; potentially valuable cryogenic procedures for crop preservation of cultured plant meristems; application of in vitro techniques in preservation of root and tuber crops; germplasm conservation of food yams; and conservation of coffee.
Part 1 Perspective: Biotechnology in conservation of genetic resources; backgrounds and principles of in vitro conservation of plant genetic resources. Part 2 Methodology: Potentially valuable cryogenic procedures for cryopreservation of cultured plant meristems; medium and longterm storage of in vitro cultures temperate fruit and nut crops; application of in vitro techniques in the preservation of conifer germplasm and in conifer tree improvement; application of in vitro techniques in preservation of root and tuber crops; cryopreservation of zygotic and somatic embryos of tropical species producing recalcitrant or intermediate seeds. Part 3 Specific crops: conservation of citrus genetic resources; cryopreservation of citrus sinesis cultured cells; applications and limitations of conserving in vitro cultures of prunus; in vitro germplasm conservation of sugarcane cultivars, basic sugarcane species and related genera; germplasm conservation of food yams (discorea spp.) - constraints, applications and future prospects; in vitro conservation of coffee (coffea spp.) germplasm stephane dussert, florent engelmman, Nathalie Chabrillange.
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