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Cryopreservation and Freeze-Drying Protocols

Handbook / Manual
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Series: Methods in Molecular Biology Volume: 1257

By: Willem F Wolkers(Editor), Harriëtte Oldenhof(Editor)

528 pages, 34 colour & 56 b/w illustrations, 25 tables


Hardback | Jan 2015 | Edition: 3 | #220977 | ISBN-13: 9781493921928
Availability: Usually dispatched within 1 week Details
NHBS Price: £98.99 $134/€112 approx

About this book

Cryopreservation and Freeze-Drying Protocols provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying.

Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.

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See the previous edition

Cryopreservation and Freeze-Drying Protocols

Cryopreservation and Freeze-Drying Protocols is a compilation of robust,...

NHBS Price: £115.50 $157/€130 approx


PART I: Fundamental Aspects And Freezing Technology
1. Principles of Cryopreservation David E. Pegg
2. Principles of Cryopreservation By Vitrification Gregory M. Fahy and Brian Wowk
3. Modeling and Optimization of Cryopreservation James Benson
4. The Principles of Freeze-Drying Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward

PART II: Methods To Study Freezing and Drying Processes
5. Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells Willem F. Wolkers and Harriette Oldenhof
6. Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations Wendell Q. Sun
7. Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy Jens O. M. Karlsson
8. Laser Scanning Microscopy in Cryobiology Frank Stracke, Asger Kreiner-Moller, and Heiko Zimmermann
9. Low Temperature Electron Microscopy Roland A. Fleck

PART III: Cryopreservation Protocols
10. Cryopreservation of Semen From Domestic Livestock Harald Sieme, Harriette Oldenhof
11. Cryopreservation of Mammalian Oocytes Victoria Keros, Barry J. Fuller
12. Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts Juergen Liebermann
13. Efficient Cryopreservation of Human Pluripotent Stem Cells By Surface-Based Vitrification Julia C. Neubauer, Axel F. Beier, Nils Geijsen, and Heiko Zimmermann
14. Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. Mcgowan, and H. Robin Tervit
15. Membrane Modification Strategies for Cryopreservation Phillip H. Purdy and James K. Graham
16. Sperm Clean-Up and Density Centrifugation Procedures Harald Sieme and Harriette Oldenhof
17. Cryopreservation of Red Blood Cells Johan W. Lagerberg
18. Cord Blood Clinical Processing, Cryopreservation, and Storage Heidi Elmoazzen and Jelena L. Holovati
19. Directional Freezing For Large Volume Cryopreservation Joseph Saragusty
20. Vitrification Of Heart Valve Tissues Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, and Lia H. Campbell
21. Cryopreservation of Plant Cell Lines Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
22. Writing Standard Operating Procedures (Sops) For Cryostorage Protocols: Using Shoot Meristem Cryopreservation As An Example Keith Harding and Erica E. Benson

PART IV: Freeze-Drying Protocols
23. Freeze-Drying Of Proteins Baolin Liu, Xinli Zhou
24. Freeze-Drying Of Lactic Acid Bacteria Fernanda Fonseca, Stephanie Cenard, Stephanie Passot
25. Freeze-Drying Of Mammalian Sperm Levent Keskintepe, Ali Eroglu
26. Freeze-Drying Of Decellularized Heart Valve Tissues Willem F. Wolkers, Andres Hilfiker

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