Dendritic Cell Protocols

About this book

Given the vital importance of immune system research, the gathering of clear, consistent, and informative protocols involving the study of dendritic cells is paramount. Bringing the popular first edition fully up to date, this volume presents protocols from experts in the field that cover the basics and more complex forays into the exploration of DC development and function, both in mice and humans.

Contents

Part I: Reviews 1. Review of Human DC subtypes Xinsheng Ju, Georgina Clark, and Derek N.J. Hart 2. Review of Murine Dendritic Cells: Types, Location, and Development Tewfik Miloud, Gunter J. Hammerling, and Natalio Garbi Part II: Human DC Protocols 3. Isolation of Human Blood DC Subtypes Andrew J. Kassianos, Sarah L. Jongbloed, Derek N.J. Hart, and Kristen J. Radford 4. Isolation of Immature Primary Langerhans Cells from Human Epidermal Skin Marein A.W.P. de Jong, Lot de Witte, and Teunis B.H. Geijtenbeek 5. Isolation and in vitro Generation of Gene-Manipulated Human Plasmacytoid and Conventional Dendritic Cells Remko Schotte, Heike Schmidlin, Maho Nagasawa, Wendy Dontje, Julien J. Karrich, Christel Uittenbogaart, Hergen Spits, and Bianca Blom 6. In vivo Modulation of Gene Expression by Lentiviral Transduction in "Human Immune System" Rag2-/-gc-/- Mice Anja U. van Lent, Mireille Centlivre, Maho Nagasawa, Julien J. Karrich, Stephan Pouw, Kees Weijer, Hergen Spits, Bianca Blom, and Nicolas Legrand 7. Generation of Stable Th1/CTL-, Th2- and Th17-inducing Human Dendritic Cells Pawel Kalinski, Eva Wieckowski, Ravikumar Muthuswamy, and Esther de Jong 8. Pharmacological Modification of Dendritic Cells to Promote Their Tolerogenicity in Transplantation Heth R. Turnquist, Ryan Fischer, and Angus W. Thomson 9. Towards a Standardized Protocol for the Generation of Monocyte-Derived Dendritic Cell Vaccines Michael Erdmann and Beatrice Schuler-Thurner Part III: Murine Dendritic Cell Methods 10. Generation of Mouse CD8+, CD8--, and Plasmacytoid Dendritic Cells Equivalents in vitro Using flt3 Ligand Shalin H. Naik, Meredith O'Keeffe, Anna Proietto, Hubertus Hochrein, Ken Shortman, and Li Wu 11. Generation of Large Numbers of pro-DCs and pre-DCs in vitro Shalin H. Naik 12. The Generation of Plasmacytoid and Conventional Dendritic Cells with M-CSF Meredith O'Keeffe, Ben Fancke, and Hubertus Hochrein 13. Isolation of Common Dendritic Cell Progenitor (CDP) from Mouse Bone Marrow Nobuyuki Onai, Markus G. Manz, and Michael A. Schmid 14. The Isolation of Mouse Dendritic Cells from Lymphoid Tissues and the Identification of Dendritic Cell Subtypes by Multiparameter Flow Cytometry David Vremec 15. Isolation of Cutaneous Dendritic Cells Julie Helft and Miriam Merad 16. Isolation of Skin Dendritic Cells from Mouse and Man Patrizia Stoitzner, Nikolaus Romani, Alexander D. McLellan, Christoph H. Tripp, and Susanne Ebner 17. Identification and Isolation of Rodent Respiratory Tract Dendritic Cells Philip A. Stumbles, Deborah H. Strickland, Mathew E. Wikstrom, Jennifer A. Thomas, Christophe von Garnier, and Patrick G. Holt 18. Isolation of Mouse Thymic Dendritic Cell Precursors Li Wu and Angela D'Amico 19. Isolation of Rat Intestinal Lymph DC Simon Milling and Gordon MacPherson 20. Mouse Models of Viral Infection: Influenza Infection in the Lung Adele M. Mount and Gabrielle T. Belz 21. DCs in Mouse Models of Intracellular Bacterial Infection Michael Neuenhahn, Matthias Schiemann, and Dirk H. Busch 22. Studying the Function of Dendritic Cells in Mouse Models of Asthma Philippe Pouliot, Monique A. Willart, Hamida Hammad, and Bart N. Lambrecht 23. Direct ex vivo Activation of T cells for Analysis of Dendritic Cell Antigen Presentation Gabrielle T. Belz 24. Methods to Study Pulmonary Dendritic Cell Migration Claudia Jakubzick and Gwendalyn J. Randolph 25. Measuring pH, ROS Production, Maturation, and Degradation in Dendritic Cell Phagosomes Using Cytofluorometry-Based Assays Ariel Savina, Pablo Vargas, Pierre Guermonprez, Ana-Maria Lennon, and Sebastian Amigorena 26. Adaptive Treg Generation by DCs and Their Functional Analysis Li Wang 27. Generation of Parabiotic Mice for the Study of DC and DC Precursor Circulation Claudia Waskow 28. Defining in vivo Dendritic Cell Functions Using CD11c-DTR Transgenic Mice Liat Bar-On and Steffen Jung

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