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British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Differential-Display Reverse Transcription-PCR (DDRT-PCR)

By: S Colonna-Romano, A Leone and B Maresca
124 pages, 23 figs
Publisher: Springer Nature
Differential-Display Reverse Transcription-PCR (DDRT-PCR)
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  • Differential-Display Reverse Transcription-PCR (DDRT-PCR) ISBN: 9783540632979 Paperback Jan 1998 Not in stock: Usually dispatched within 1-2 weeks
    £89.99
    #95751
Price: £89.99
About this book Contents Customer reviews Related titles

About this book

Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA.Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

Contents

Abbreviations; Overview; Preparation of Total RNA; Differential Display; Size Separation of cDNA Fragments; Isolation of Differentially Expressed cDNA Fragments; Reamplification of Eluted cDNA; Cloning of Amplified cDNA Fragments; Checking Subcloned Fragments; Confirmation of Differential Expression of Cloned cDNA Fragments; Northern Blot Affinity Capturing of cDNA; Sequencing of Differentially Expressed cDNA Fragments; References; Appendix: Materials, Equipment, Reagents, and Suppliers; Subject Index.

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By: S Colonna-Romano, A Leone and B Maresca
124 pages, 23 figs
Publisher: Springer Nature
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