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DNA Topoisomerases Protocols, Volume 1: DNA Topology & Enzymes

Handbook / Manual

Series: Methods in Molecular Biology Volume: 1

Edited By: Mary-Ann Bjornsti and Neil Osheroff

Humana Press

Hardback | Dec 1998 | #86816 | ISBN: 0896034445
Availability: Usually dispatched within 1 week Details
NHBS Price: £108.00 $146/€122 approx

About this book

DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems. invaluable practical aid. The central and expanding role of these enzymes in basic cell biology together with their important pharmaceutical applications will ensure that book will remain relevant for some years to come. - Cell Biology International 2000 The introductory chapter by the editors gives a beautifully brief and concise overview of the entire family of DNA topoisomerases and related enzymes and can be used as an Ariadne's thread to the key papers of the field. Another useful item is the appended compendium of topoisomerase sequences, which is likely to be helpful in designing expression vectors, deletion mutants, etc. Most of the other chapters contain a lot of useful information and easily followed procedures.a very valuable addition to the topoisomerase literature. It summarizes a lot of useful information for those engaged or getting engaged in research on DNA topology or topoisomerases. Those just wanting to carry out some of the analytical procedures will in most cases find what they need, i.e. a concise delineation of the analytical background, some detailed and easy to follow protocols, and a comprehensive guideline for the interpretation of the results. As such, the book definitely deserves a place on many laboratory shelves - FEBS Letters The introductory chapter is one of the most concise and clearly presented summaries of our current knowledge that has been written, and it will be useful even for experts in the field. The volume concludes with a well-organized and useful compendium of toposomerase DNA sequences. Volume I is clearly a valuable source of methodological expertise for anyone studying topoisomerases. - New Biological Books We recommend greatly this remarkable book to all our readers, without exception. - Cellular and Molecular Biology


Overview Neil Osheroff and Mary-Ann Bjornsti. Assaying DNA Topoisomerase I Relaxation Activity Lance Stewart and James J. Champoux. Assaying DNA Topoisomerase II- Catalyzed DNA Relaxation and Catenation Neil Osheroff. DNA Topoisomerase II Catalyzed DNA Decatenation Andrea Haldane and Daniel Sullivan. Plasmid DNA Supercoiling by DNA Gyrase Penny Sayer Martin L. Goble Mark Oram and L. Mark Fisher. Analyzing Reverse Gyrase Activity Michel Duguet Christine Jaxel Anne Ceile Declais Fabrice Confalonieri Janine Marsualt Claire Bouthier de la Tour Marc Nadel and Christine Portemer. Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography Paul S. Kingma John M. Fortune and Neil Osheroff. Use of Real-Time Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II Janet E Lindsley. Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Band Shift Assay Stewart Shuman. Filter Binding Assays for Topoisomerase-DNA Complexes Joaquim Roca. DNA Topoisomerase I-Mediated Nicking of Circular Duplex DNA James J. Champoux. DNA Topoisomerase II-Mediated Plasmid DNA Cleavage Neil Osheroff. DNA Topoisomerase I-Induced Cleavage of Linear DNA Substrates Mary-Ann Bjornsti. Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives Kenneth N. Kreuzer Catherine H. Freudenreich and Yves98-015 Pommier. DNA Topoisomerase II-Induced Cleavage of Linear DNA Substrates and Analysis of Cleavage Sites Yves Pommier. Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation Anni H. Anderson Kent Christiansen and Ole Westergaard. Synthesis and Use of DNA containing a 5'-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases Alex B. Burgen Jr. DNA Topoisomerase II-Mediated Religation of DNA Neil Osheroff. Isolation of Covalent Enzyme-DNA Complexes Thomas C. Rowe Dale Grabowski and Ram Ganapathi. Alkaline and Neutral Elution Kurt Kohn. ICE Bioassay: Isolating In Vivo Complexes of Enzyme to DNA Deepa Subramanian Christine Sommer Furbee and Mark T. Muller. Drug Unwinding Test Using Eukaryotic DNA Topoisomerase I Linus L. Shen. Drug-DNA Interactions David Graves. Quinolone Interactions with DNA and DNA Gyrase Linus L. Shen. Bactericidal Assays for Fluoroquinolones Thomas D. Gootz. Drug Toxicity in E coli Cells Expressing Human DNA Topoisomerase I Scott T. Taylor and Rolf Menzel. Establishing the Specificity of DNA Topoisomerase-Targetted Drugs in Yeast John Nitiss and Mary-Ann Bjornsti. Selection of Drug-Resistant DNA Topoisomerase I Mutants: Yeast Mary-Ann Bjornsti and Piero Benedetti. Selection of Drug-Resistant DNA Topoisomerase II Mutants: Yeast John Nitiss. Drug-Induced Cytotoxicity in Tissue Culture Cynthia E. Herzog and Leonard A. Zwelling. Analysis of Drug Uptake-Flow Cytometry Grace K. Zoorob and Thomas G. Burke. Cell-Cycle Analysis of Drug-Treated Cells Frank Traganos Gloria Juan and Zeigniew Darzynkiewicz. Assaying Drug-Induced Apoptosis Zeigniew Darzynkiewicz Gloria Juan and Frank Traganos. Selection of Drug-Resistant Mutants: Mammalian Cells William Beck. Single- Strand Conformational Polymorphism Analysis of DNA Topoisomerase I and II Mary K. Danks. PCR-Based Cloning of DNA Topoisomerase Genes Wai-Mun Huang. Future Goals James C. Wang.

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