Early integration is the key to success in industrial biotechnology. This is as true when a selected wild-type organism is put to work as when an organism is engineered for a purpose.
The present volume Engineering and Manufacturing for Biotechnology took advantage of the 9th European Congress on Biotechnology (Brussels, Belgium, July 11-15, 1999): in the topics handled and in the expertise of the contributors, the engineering science symposia of this congress offered just what was needed to cover the important topic of integration of process engineering and biological research.
The editors have solicited a number of outstanding contributions to illustrate the intimate interaction between productive organisms and the numerous processing steps running from the initial inoculation to the packaged product. Upstream processing of the feed streams, selection of medium components, product harvesting, downstream processing, and product conditioning are just a few major steps. Each step imposes a number of important choices. Every choice is to be balanced against time to market, profitability, safety, and ecology.
Editors Preface. Part I: Upstream processes and fermentation. Pretreatment processes of molasses for the utilization in fermentation processes; G. Calik, et al. Lactic acid fermentation of hemicellulose liquors and their activated carbon pretreatments; J. Perttunen, et al. Enzymic solubilisation of proteins from tropical tuna using alcalase and some biological properties of the hydrolysates; F. Guerard, et al. Influence of the experimental conditions on the hydrolysis process in fish hydrolysates; R. Ravallec-Ple, et al. Part II: Process Modelling. Mathematical modelling of microbial processes -- Motivation and means; T. Agger, J. Nielsen. Macroscopic modelling of bioprocesses with a view to engineering applications; Ph. Bogaerts, R. Hanus. A Model discrimination approach for data analysis and experimental design; R. Takors, et al. Model based sequential experimental design for bioprocess optimisation -- An overview; R. Berkholz, R. Guthke. Metabolic flux modelling as a tool to analyse the behavior of a genetically modified strain of Saccharomyces cerevisiae; J.M. Urrieta-Saltijeral, et al. Metabolic investigation of an anaerobic cellulolytic bacterium: Fibrobacter succinogenes; C. Creuly, et al. Part III: Integrated Processes. Crossflow ultrafiltration of Bacillus licheniformis fermentation medium to separate protease enzymes; S. Takac, et al. Part IV: Monitoring and Control. Evaluating KLa during fermentation using many methods simultaneously; K. Pouliot, et al. Respiration quotient: estimation during batch cultivation in bicarbonate buffered media; R. Neeleman. Fermentation phase detection using fuzzy clustering techniques and neural networks for improved control; T.K. Hamrita, S. Wang. Simulation, design and model based predictive control of photobioreactors; J.-F. Cornet, et al. Part V: Reactor Engineering. Bioreactors for space: biotechnology of the next century; I. Walther, et al. Part VI: Immobilisation and Permeabilisation. State of the art developments in immobilised yeast technology for brewing; C.A. Masschelein, J. Vandenbussche. Immobilized yeast bioreactor systems for brewing -- Recent achievements; V.A. Nedovic, et al. New matrices and bioencapsulation processes; U. Jahnz, et al. Part VII: Downstream Processing. Industrial downstream processing; M. Laustsen. Separation of a-lactalbumin and b-lactoglobulin by preparative chromatography using simulated moving beds; S.L. Lucena, et al. High-speed pectic enzyme fractionation by immobilised metal ion affinity membranes; S.A. Camperi, et al. Part VIII: Economic finalities. Economic benefits of the application of biotechnology -- Examples; M. Etschmann, et al. Enzyme stability and stabilisation: applications and case Studies; G.A. Drago, T.D. Gibson. Improvements of enzyme stability and specificity by genetic engineering; M. Pohl, M.-R. Kula. An approach to desiccation-tolerant bacteria in starter culture production; F. Weekers, et al. Biotechnological research and the dairy industry; H. Neubauer, B. Mollet. Immobilised cell technology in winery and fruit wine production; R. Cachon, C. Divies. A new polysaccharide derived from plant rhizosphere: production, purification and physico-chemical properties; J.M. Crompin, et al. Initiation, growth and immobilisation of cell cultures of Taxus spp. for placlitaxel production; C.W. Tang, et al. Effective biofuel production by an intelligent bioreactor; H. Fukuda, et al. Part IX: Patents and Licenses. Translating European biotech into US patents do's, don'ts, & costs; T.M. Saunders. Index.
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