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Epithelial Cell Culture Protocols: Methods and Protocols

Handbook / Manual

Series: Methods in Molecular Biology Volume: 945

By: Scott H Randell(Editor), M Leslie Fulcher(Editor)

481 pages, 35 colour & 47 b/w illustrations, 20 tables

Humana Press

Hardback | Oct 2012 | Edition: 2 | #205825 | ISBN-13: 9781627031240
Availability: Usually dispatched within 1 week Details
NHBS Price: £108.00 $139/€118 approx

About this book

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few.

Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.


1. Progress Towards Drosophila Epithelial Cell Culture Amanda Simcox
2. Culture of Choriod Plexus Epithelial Cells and In Vitro Model of Blood-CSF Barrier Andrew D. Monnot and Wei Zheng
3. Methods for Culture of Human Corneal and Conjunctival Epithelia Sandra J. Spurr-Michaud and Ilene K. Gipson
4. The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye Timothy A. Blenkinsop, Enrique Salero, Jeffrey Stern, and Sally Temple
5. Oral and Pharyngeal Epithelial Keratinocyte Culture Kantima Leelahavanichkul and J. Silvio Gutkind
6. Primary Culture of the Human Olfactory Neuroepithelium Nancy E. Rawson and Mehmet Hakan Ozdener
7. Primary Culture of Mammalian Taste Epithelium Mehmet Hakan Ozdener and Nancy E. Rawson
8. Human Nasal and Tracheo-Bronchial Respiratory Epithelial Cell Culture M. Leslie Fulcher and Scott H. Randell
9. Culture and Differentiation of Mouse Tracheal Epithelial Cells Yingjian You and Steven L. Brody
10. Isolation and Culture of Alveolar Epithelial Type I and Type II cells from Rat Lungs Robert F. Gonzalez and Leland G. Dobbs
11. Classical Human Epidermal Keratinocyte Cell Culture Cathy Rasmussen, Christina Thomas-Virnig, and B. Lynn Allen-Hoffmann
12. Mouse Epidermal Keratinocyte Culture Luowei Li
13. Three Dimensional Culture of Human Breast Epithelial Cells: The How and The Why Pierre-Alexandre Vidi, Mina J. Bissell, and Sophie A. Lelievre
14. Three Dimensional Cultures of Mouse Mammary Epithelial Cells Rana Mroue and Mina J. Bissell
15. Isolation, Characterisation and Re-aggregate Culture of Thymic Epithelial Cells Natalie Seach, Maree Hammett, and Ann Chidgey
16. Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development Akihide Kamiya and Hiromitsu Nakauchi
17. Long Term Culture and Co-Culture of Primary Rat and Human Hepatocytes Maria Shulman and Yaakov Nahmias
18. Tissue-Culture Correlational Study of Genetic Cholangiopathy of Autosomal Recessive Polycystic Kidney Disease Yasuni Nakanuma, Yasunoi Sato, and Kenichi Harada
19. Primary Mouse Small Intestinal Epithelia Cell Cultures Toshiro Sato and Hans Clevers
20. Isolation, Growth and Characterization of Human Renal Epithelial Cells Using Traditional and 3D Methods J.J. Gildea, H.E. McGrath, R. E. VanSciver, Wang D. Bigler, and Robin A. Felder
21. Human Female Reproductive Tract Epithelial Cell Culture Rachel W. S. Chan, Abby S. C. Mak, William S. B. Yeung, Kai-Fai Lee, Annie N. Y. Cheung, Hextan Y. S. Ngan, and Alice S. T. Wong
22. Primary Culture and Propagation of Human Prostate Epithelial Cells Birunthi Niranjan, Mitchell G. Lawrence, Melissa M. Papargiris, Michelle G. Richards, Shirin Hussain, Mark Frydenberg, John Pedersen, Australian Prostate Cancer BioResource, Renea A. Taylor, and Gail P. Risbridger
23. Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Structures Yuanyuan Zhang and Anthony Atala
24. Analysis of Tissue Interactions in Ectodermal Organ Culture Pauliina M. Munne, Katja Narhi, and Frederic Michon
25. Generation of Human Cell Lines Using Lentiviral-Mediated Genetic Engineering Patrick Salmon
26. Viral Transformation of Epithelial Cells Jennifer A. Regan and Laimonis A. Laimins

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