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About this book
Martin Tymms has created a powerful collection of key techniques for the study of those DNA sequences and protein factors that regulate the transcription of protein encoding genes. This practical compendium includes not only well established protocols, but also novel techniques that are now being widely adopted. Among the important new methods treated are the use of triplex-forming oligonucleotides, the application of whole genome PCR to the isolation of gene promoters/enhancers, the analysis of in vivo methylation, and in vivo footprinting using UV light and ligation-mediated PCR. Transcription Factor Protocols provides both experienced workers and new researchers with a vital first-stop reference for all those exploring the role of transcription factors in gene regulation today.
Contents
Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method, Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, and Arun Seth. In Vivo Footprinting Using UV Light and Ligation-Mediated PCR, Gerd P. Pfeifer and Stella Tommasi. Identification of DNaseI Hypersensitive Sites Within Nuclei, Peter N. Cockerill. Analysis of In Vivo Methylation, Hans-Henrik M. Dahl and Wendy M. Hutchison. Detection of Transcription Factor Partners with a Yeast One Hybrid Screen, Michael Sieweke. Inverse PCR (IPCR) for Obtaining Promoter Sequence, Tony Triglia. PCR-Directed Linker Scanning Mutagenesis, Kurt Gustin and Robert D. Burk. Transfection Technologies, Elaine T. Schenborn. The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues, Charles M. Nicolet and Ning-Sun Yang. Optimizing Electroporation Conditions for the Transformation of Mammalian Cells, William C. Heiser. Calcium Phosphate Transfection of Mammalian Cultured Cells, Elaine T. Schenborn and Virginia Goiffon. DEAE-Dextran Transfection of Mammalian Cultured Cells, Elaine T. Schenborn and Virginia Goiffon. Liposome-Mediated Transfection of Mammalian Cells, Elaine T. Schenborn and Jennifer Oler. Assays for Transcriptional Activity Based on the Luciferase Reporter Gene, S. Roy Himes and M. Frances Shannon. Transient Transfection of Schneider Cells in the Study of Transcription Factors, Guntram Suske. Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription, Marina Kochetkova and M. Frances Shannon. Expression and Purification of Histidine-Tagged Transcription Factors, Donna R. Cohen. Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein, Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel. Electrophoretic Mobility Shift Assays, Peter L. Molloy. In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors, Martin J. Tymms. In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors, Fujiko Watt. Computer Software of Eukaryotic Promoter Analysis, Dan S. Prestridge. Index.
Customer Reviews
Handbook / Manual
Edited By: Martin J Tymms
350 pages
Transcription Factor Protocols, volume 130 of Humana Press' popular Methods in Molecular Biology series, aids in the study of transcription factors by providing a compilation of methodologies for the study of the complex interactions between transcription factors, proteins, and DNA. Like the other volumes in this series, the techniques in Transcription Factor Protocols are contributed by an international group of scientists well experienced in the methodologies presented the detailed information provided in Transcription Factor Protocols makes it a welcome addition to the reference collection of an academic library that serves scientists who are conducting molecular biology research. It is well representative of the previous volumes in the Methods in Molecular Biology series-Cecelia Brown reviewing for E-Streams "...This book would be a worthwhile addition to the library of any researcher in the general field of transcription regulation...Each of the methods described is accompanied by a well written introductory section setting it in context. The descriptions of the protocols themselves are concisely written in step by step fashion, and readily comprehensible. Recipes for all buffers, with plentiful footnotes describing potential pitfalls, are provided. All chapters end with a detailed bibliography..."-Cell Biology International 2000 "...offers a very complete and up-to-date collection of the techniques needed to explore important aspects in this field...Twenty-two chapters include essential prerequisites, with a clear and extensive coverage of transfection technologies, as well as novel techniques such as the isolation of regulatory DNA sequences by whole genome PCR, functional characterization of such DNA sequences by the use of triplex-forming oligonucleotides, or in vivo footprinting using UV light and ligation-mediated PCR for the identification of protein-bound DNA sequences. The chapters are clearly written by knowledgeable investigators with extensive personal experience of the techniques that the describe. As in previous volumes of the Methods in Molecular Biology series, very detailed step-by-step procedures are provided. Many notes and practical tips are helpful to researchers unfamiliar with the field or new to the most recent and complex strategies." - Cell Biology and Function "...a book of great importance and which should be in the hands of all cellular and molecular biologists." - Cellular and Molecular Biology
