Expression Systems Methods Express

About this book

Expression Systems: Methods Express is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. This book is an essential laboratory manual for all life science researchers who use expression systems.

Contents

1. Expression strategy Michael Dyson 2. Protein expression in Escherichia coli Rosalind Kim, Lawrence Berkeley National Laboratory 3. Expression engineering of synthetic antibodies using ribosome display Lydia M. Contreras Martinez and Matthew DeLisa, both at Cornell University 4. Refolding proteins from inclusion bodies Renaud Vincentelli, Methods in Structural Genomics, Marseilles 5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters Geoffrey Waldo and Stephanie Cabantous, both at Los Alamos National Laboratory 6. Protein expression in the wheat germ cell-free system Yaeta Endo and Tatsuya Sawasaki, both at Ehime University 7. Saccharomyces cerevisiae; A microbial eukaryotic expression system Christine Lang, Technische Universitat Berlin 8. Expression of proteins in Pichia pastoris Geoff and Joan Lin-Cereghino and Wilson Leung, all at University of the Pacific 9. Improved baculovirus expression vectors Linda King, Richard Hitchman and Robert Possee, all at Oxford Brookes University 10. Transient transfection of insect cells for rapid expression screening and protein production Robert Novy, Kathryn Loomis, Courtney Rockwell, Heather Sternard and Keith Yaeger, all at EMD Biosciences, Inc. 11. Generation of stable CHO cell lines for protein expression Zhijian Lu, Haley Laken, Jimin Zhang, Xiaotian Zhong and Richard Zollner, all at Wyeth 12. Transient expression in HEK293-EBNA1 cells Yves Durocher, Roseanne Tom and Louis Bisson, all at NRC 13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria Oscar Kuipers and Jan Kok, both at University of Groningen 14. Protein expression using lentiviral vectors Bernard Massie, Renald Gilbert and Sophie Broussau, all at Biotechnology Research Institute 15. Expression in mammalian cells using BacMam viruses Yu-Chen Hu and Hsiao-Ping Lee, both at National Tsing Hua University, Taiwan List of suppliers Index

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