Fundamentals of Light Microscopy and Electronic Imaging

About this book

This book provides a coherent introduction to the principles and applications of the integrated electron microscope system. It is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

Contents

Chapter 1: Fundamentals of Light Microscopy
Chapter 2: Light and Color
Chapter 3: Illuminators, Filters, and the Isolation of Specific Wavelengths
Chapter 4: Lenses and Geometrical Optics
Chapter 5: Diffraction and Interference in Image Formation
Chapter 6: Diffraction and Spatial Resolution
Chapter 7: Phase Contrast Microscopy and Darkfield Microscopy
Chapter 8: Properties of Polarized Light
Chapter 9: Polarization Microscopy
Chapter 10: Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy
Chapter 11: Fluorescence Microscopy
Chapter 12: Fluorescence Imaging of Dynamic Molecular Processes
Chapter 13: Confocal Laser Scanning Microscopy
Chapter 14: Two-Photon Excitation Fluorescence Microscopy
Chapter 15: Superresolution Imaging
Chapter 16: Imaging Living Cells With The Microscope
Chapter 17: Fundamentals of Digital Imaging
Chapter 18: Digital Imaging Processing

Appendix I
Appendix II
Appendix III

Glossary
References
Index

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