Thirty protocols by leading researchers describe in detail all the essential molecular methods for working with gene transfer systems, along with the methods for gene transfer to specific tissue types either in vivo or ex vivo. The easily reproducible methods range from those for specific viral and nonviral for both genetic and acquired diseases, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor supressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.
Such a compilation is of great interest fro researchers who get interested in the protocols utilized in gene transfer and in thier applications. Furthermore, for researchers already involved in gene therapy, this book is a very good reference with a note section containing many details and comments, useful to improve current protocols. - ACTA Neurologica Belgica
Methods for Gene Transfer Using DNA-Adenovirus Conjugates. Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors. Generation of High Titer Helper-Free Retroviruses by Transient Tranfection. Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers. Development of Replication-Defective Herpes Simplex Virus Vectors. Methods for the Use of Poliovirus Vectors for Gene Delivery. Methods for the Construction of Human Papillomavirus Vectors. Methods for Liposome-Mediated Gene Transfer to the Arterial Wall. Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes. Methods for Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium. Methods for Adenovirus-Mediated Gene Transfer to Airway Epithelium. Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung. In Situ Retrovirus-Mediated Gene Transfer into the Liver. Methods for Delivery of Genes to Hepatocytes in Vivo Using Recombinant Adenovirus Vectors. Methods for Producing High-Titer Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells. Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells. Methods for Retrovirus- Mediated Gene Transfer into Primary T-Lymphocytes. Methods for Retrovirus-Mediated Gene Transfer to CD34+ Enriched Cells. Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy. Methods for Particle-Medicated Gene Transfer into Skin. Methods for Liposome- Mediated Gene Transfer to Tumor Cells in Vivo. Methods for Retrovirus-Mediated Gene Transfer to Tumor Cells. Cationic Liposome-Mediated Gene Transfer to Tumor Cells In Vitro and In Vivo. Methods for Use of Cytokine Gene-Modified Tumor Cells in Immunotherapy of Cancer. Methods for Generation of Genetically Modified Fibroblasts for Immunotherapy of Cancer. Methods for Gene Transfer to Synovium. Methods for Adenovirus-Mediated Gene Transfer to Synovium in Vivo. Methods for Use of Stromal Cells for Therapeutic Gene Therapy. Suppression of the Human Carcinoma Phenotype by an Antioncogene Ribozyme. Methods for Cancer Gene Therapy using Tumor Suppressor Genes.
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