784 pages, 74 color line illus. and 195 HT line illus.
The Handbook of Biomedical Nonlinear Optical Microscopies provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians.
The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
It should be in all University librares...this book, with chapters on theory and practice, will be very useful for the next-even this-generation of cell imaging, and we can all expect to be delighted with the aesthetics as well as the utility of the results! Jack Cohen Biologist
PART I. HISTORICAL DEVELOPMENT OF NONLINEAR OPTICAL SPECTROSCOPY AND MICROSCOPY; INTRODUCTION TO PART I: HISTORICAL DEVELOPMENT OF NONLINEAR OPTICAL MICROSCOPY AND SPECTROSCOPY; Chapter 1 The Genesis of Nonlinear Optical Microscopies and their Impact on Modern Developments; Chapter 2 The Scientific Life of Maria Goppert-Mayer; Chapter 3 The History of Perturbation Theory from Astronomy to Quantum Mechanics; Chapter 4 English Translations of and Translator's Notes on Maria Goppert-Mayer's Theory of Two-Quantum Processes; PART II. NONLINEAR OPTICAL SPECTROSCOPY; INTRODUCTION TO PART II: NONLINEAR OPTICAL SPECTROSCOPY; Chapter 5 Classical and Quantum Theory of One-Photon and Multiphoton Fluorescence Spectroscopy; Chapter 6 Second- and Higher-Order Harmonic Generation; Chapter 7 Theory of Spontaneous and Coherent Raman Scattering; PART III. NONLINEAR OPTICAL INSTRUMENTS FOR MICROSCOPIC IMAGING AND ANALYSIS; INTRODUCTION TO PART III: NONLINEAR OPTICAL INSTRUMENTS FOR MICROSCOPIC IMAGING AND ANALYSIS: REVIEW AND FORECAST; Chapter 8 Laser Sources for Non-Linear Microscopy; Chapter 9 Ultrashort Optical Pulse Delivery for Nonlinear Optical Microscopy; Chapter 10 An Optical Design Primer for Nonlinear Optical Microscopy; Chapter 11 Image Formation in Multiphoton Fluorescence Microscopy; Chapter 12 Signal Detection and Processing in Nonlinear Optical Microscopes; Chapter 13 Multiphoton Excitation of Fluorescent Probes; Chapter 14 Multiphoton-Induced Cell Damage; Chapter 15 Applications of Second-Harmonic Generation Microscopy; Chapter 16 Second-Harmonic Generation Imaging Microscopy of Structural Protein; Chapter 17 Coherent Anti-Stokes Raman Scattering (CARS) Microscopy: Instrumentation and Applications; Chapter 18 High-speed Imaging Using Multiphoton Excitation; Chapter 19 Nonlinear Multi-Spectral Optical Imaging Microscopy: Concepts, Instrumentation, and Applications; Chapter 20 Multiphoton Polarization Microscopy; Chapter 21 Lifetime-Resolved Imaging in Nonlinear Microscopy; Chapter 22 Forster Resonance Energy Transfer (FRET); Chapter 23 Two-Photon Forster Resonance Energy Transfer (FRET) Microscopy; Chapter 24 Diffraction Unlimited Far-Field Fluorescence Microscopy; Chapter 25 Two-Photon Fluorescence Correlation Spectroscopy; Chapter 26 Photobleaching and Recovery with Nonlinear Microscopy; Chapter 27 Femtosecond Laser Nanoprocessing; PART IV. BIOMEDICAL APPLICATIONS OF NONLINEAR OPTICAL MICROSCOPY; INTRODUCTION TO PART IV: BIOMEDICAL APPLICATIONS OF NONLINEAR OPTICAL MICROSCOPY; Chapter 28 Pioneering Applications Of Two-Photon Microscopy To Mammalian Neurophysiology: Seven Case Studies; Chapter 29 Applications of Non-Linear Intravital Microscopy in Tumor Biology; Chapter 30 Immunology Based on Nonlinear Optical Microscopy; Chapter 31 Multiphoton Imaging in Animal Development; Chapter 32 Nonlinear Microscopy Applied to Dermatology; Chapter 33 Cellular Metabolism Monitored by NAD(P)H Imaging with Two-Photon Excitation Microscopy
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