Handbook of Methods in Aquatic Microbial Ecology

About this book

First comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology.

Contents

Introduction (J.E. Hobbie). Isolation of Living Cells: Isolation and Enumeration of Anaerobic and Microaerophilic Bacteria in Aquatic Habitats (M.J. Ferrara-Guerrero, D.G. Marty, and A. Bianchi). Isolation and Culture of Hyperthermophilic Bacteria from Marine and Freshwater Habitats (J.A. Baross). Isolation of Psychrophilic Bacteria (R.Y. Morita). Isolation and Characterization of Bacteriocytes from a Bivalve-Sulfur Bacterium Symbiosis (S.C. Hand and A.E. Anderson). General Techniques for the Isolation and Culture of Marine Protists from Estuarine, Littoral, Psammolittoral, and Sublittoral Waters (J.J. Lee). Long-Term Culture of Marine Benthic Protists (P.G. Carey). Behavior and Bioenergetics of Anaerobic and Microaerobic Protists (B.J. Finlay). Culturing Free-Living Marine Phagotrophic Dinoflagellates (E.J. Lessard). Enrichment, Isolation, and Culture of Free-Living Heterotrophic Flagellates (D.A. Caron). Determination of Pressure Effects on Flagellates Isolated from Surface W aters (C.M. Turley). Isolation, Cloning, and Axenic Culturing of Marine Ciliates (A.T. Soldo and S.A. Brickson). Isolation and Laboratory Culture of Marine Oligotrichous Ciliates (D.J. Gifford). Extraction of Protists in Aquatic Sediments via Density Gradient Cetrifugation (D.M. Alongi). Identification, Enumeration, and Diversity: Statistical Analysis of Direct Counts of Microbial Abundance (D.L. Kirchman). Enumeration and Isolation of Viruses (C.A. Suttle). Total Count of Viruses in Aquatic Environments (G. Bratbak and M. Heldal). Improved Sample Preparation for Enumeration of Aggregated Aquatic Substrate Bacteria (M.I. Velji and L.J. Albright). Direct Estimates of Bacterial Numbers in Seawater Samples without Incurring Cell Loss Due to Sample Storage (C.M. Turley). Total and Specific Bacterial Counts by Simultaneous Staining with DAPI and Fluorochrome-Labeled Antibodies (K.A. Hoff). Use of RFLPs for the Comparison of Marine Cyanobacteria (S.E. Douglas). Use of High-Resolution Flow Cytometry to Determine the Activity and Distribution of Aquatic Bacteria (D.K. Button and B.R. Robertson). Phytoplankton Analysis Using Flow Cytometry (R.J. Olson, E.R. Zettler, and M.D. DuRand). Enumeration of Phototrophic Picoplankton by Autofluorescence Microscopy (E.A. MacIsaac and J.G. Stockner). Estimating Cell Concentration and Biomass of Autotrophic Plankton Using Microscopy (B.C. Booth). Preservation and Storage of Samples for Enumeration of Heterotrophic Protists (E.B. Sherr and B.F. Sherr). Staining of Heterotrophic Protists for Visualization via Epifluorescence Microscopy (E.B. Sherr, D.A. Caron, and B.F. Sherr). A Quantitative Protargol Stain (QPS) for Ciliates and Other Protists (D.J.S. Montagnes and D.H. Lynn). Preparation of Pelagic Protists for Electron Microscopy (B.S.C. Leadbeater). A Rapid Technique for the Taxonomy of Methanogenic Bacteria: Comparison of the Methylreductase Subunits (P.E. RouviTre and C.H. Kuhner). Extraction of DNA from Soils and Sedim ents (R.M. Atlas). D

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