Host-Bacteria Interactions Methods and Protocols

About this book

Host-Bacteria Interactions: Methods and Protocols details cutting edge protocols that cover aspects of the investigation of host bacteria interactions using mammalian and novel non mammalian infection models, cell biology, OMICS and bacterial genetics. Chapters focus on techniques that can be used to investigate different aspects of the physiopathology of bacterial infections, from the whole animal to tissue, cellular and molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Host-Bacteria Interactions: Methods and Protocols provide researchers with a comprehensive account of the practical steps necessary for carrying out each protocol successfully.

Contents

Part I: Infection Models to Study Bacterial Virulence
1. Galleria mellonella as an Infection Model for Select Agents Nicolas Sprynski, Eric Valade, and Fabienne Neulat-Ripoll
2. Drosophila as a Model for Intestinal Infections Matthieu Lestradet, Kwang-Zin Lee, and Dominique Ferrandon
3. Zebrafish Embryos as a Model to Study Bacterial Virulence Jennifer Mesureur and Annette C. Vergunst
4. Studying Host-pathogen Interaction Events in Living Mice Visualized in Real Time using Bio photonic Imaging Gary Splitter, Jerome Harms, Erik Petersen, Diogo Magnani, Marina Durward, Gireesh Rajashekara, and Girish Radhakrishnan
5. Intravital Two photon Imaging to Understand Bacterial Infections of the Mammalian Host Ferdinand X. Choong and Agneta Richter-Dahlfors

Part II: Cellular Aspects of Host-Bacteria Interactions
6. Cre Reporter Assay for Translocation (CRAfT), A Tool for the Study of Protein Translocation into Host Cells Amke den Dulk-Ras, Annette Vergunst, and Paul J.J. Hooykaas
7. Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu Monique Barel and Alain Charbit
8. Hijacking the Host Proteasome for the Temporal Degradation of Bacterial Effectors Tomoko Kubori, Andree M. Hubber, and Hiroki Nagai
9. Life Cell Imaging of Phosphoinositide Dynamics during Legionella Infection Stephen Weber and Hubert Hilbi
10. Investigating Interference with Apoptosis Induction by Bacterial Proteins Hua Niu and Yasuko Rikihisa
11. Bimolecular Fluorescence Complementation for Imaging Protein Interactions in Plant Hosts of Microbial Pathogens Lan-Ying Lee and Stanton B. Gelvin
12. Investigating TLR Signalling Responses in Murine Dendritic Cells upon Bacterial Infection Suzana Pinto Salcedo and Lena Alexopoulou

Part III: OMICS and Large Scale Screening
13. siRNA Screens using Drosophila Cells to Identify Host Factors Required for Infection Aseem Pandey, Sheng Li Ding, Thomas A. Ficht, and Paul de Figueiredo
14. Purification of Intracellular Bacteria: Isolation of Viable Brucella abortus from Host Cells Esteban Chaves-Olarte, Pamela Altamirano-Silva, Caterina Guzman-Verri, and Edgardo Moreno
15. RNA Sequencing of FACS-sorted Immune Cell Populations from Zebrafish Infection Models to Identify Cell Specific Responses to Intracellular Pathogens Julien Rougeot, Ania Zakrzewska, Zakia Kanwal, Hans J. Jansen, Herman P. Spaink, Annemarie H. Meijer
16. Taking the Shortcut for High-throughput Shotgun Proteomic Analysis of Bacteria Erica Marie Hartmann, Francois Allain, Jean-Charles Gaillard, Olivier Pible, and Jean Armengaud
17. Comparative Genomic Analysis at the PATRIC, a Bioinformatic Resource Center Alice R. Wattam, Joseph L. Gabbard, Maulik Shukla, and Bruno W. Sobral

Part IV: Approaches for Difficult Bacteria
18. A Markerless Deletion Method for Genetic Manipulation of Burkholderia cenocepacia and Other Multi Antibiotic Resistant Gram-negative Bacteria Daniel F. Aubert, Mohamad A. Hamad, and Miguel A. Valvano
19. Gene Inactivation in Coxiella burnetii Paul A. Beare and Robert A. Heinzen
20. A Chemical Mutagenesis Approach to Identify Virulence Determinants in the Obligate Intracellular Pathogen Chlamydia trachomatis Bidong Nguyen and Raphael Valdivia

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