Imaging Living Cells

About this book

Improved technology for imaging living cells, specific cellular targets and organelles is having a dramatic impact on basic and applied research. By combining optical design and molecular genetics, a new series of tools is being developed and successfully applied together with classical probes. Novel labelling strategies, better software for image enhancement and analysis are now available and allow image acquisition with greater speed and precision. This lab manual, intended as for bench-top use, is suitable for both scientists and graduate students, combines an update on the most advanced imaging procedures with detailed protocols. Examples, cleverly selected from the wide repertoire of cell pyhsiology, cover different functional aspects such as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.

Contents

Strategies for Quantitative Digital Imaging of Biological Activity with Ion-Sensitive Fluorescent Probes * High Resolution 3-D Imaging by Image Restoration * Imaging Calcium in the Cytoplasm and in Organelles with Fluorescent Dyes * Confocal Calcium Imaging * Imaging Whole Organs * Simultaneous Measurement of Membrane Current and Intracellular Calcium * Quantitative Calcium Imaging in Brain Slices * Common Methods for Measuring Apoptotic Cell Death with Flow Cytometry * Expressing, Targeting, and Calibrating Recombinant Aequorin * Analysis of Regulated Gene Expression by Microinjection and Digital Luminescence Imaging of Single Living Cells * The New Green Fluorescent Protein Mutants and Their Applications * Imaging Green Fluorescent Protein in Transgenic Plants.

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