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About this book
This laboratory manual will be welcomed by all research scientists involved in the extraction, fractionation and isolation of compounds from natural materials, especially those working with plants. This book is clear and concise, and features practical exercises to illustrate the techniques described in every chapter. It will provide an invaluable research reference tool for those scientists investigating the potential benefits of ethnomedicine and the properties of chemicals isolated from natural flora.
Contents
Important concepts; methods for extraction and sample clean-up; crude fractionation procedures; chromatographic fractionation procedures; chromatographic procedures using column and liquid-liquid systems; layer chromatography; analysis of crude extracts, fractions and isolated compounds; general methods; practical exercises in chemical screening and fractionation of crude extracts from plant materials. Appendix: recipies for chemical test reagents.
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