About this book
The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of: history and background; principles and theory; equipment and reagents; protocols; troubleshooting; applications; improvements; results; comparisons with other methods; and, future prospects and developments. This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.
Contents
Introduction, G.R. Taylor Arbitrarily Primed PCR Fingerprints, F. Mathieu-Daude, D. Ralph, and M. McClelland Minisatellite Analysis, G. Rysiecki, W.P. Childs, and N. Duxbury Microsatellite Analysis, S. Payne ARMS Analysis of Point Mutations, S. Little Solid Phase Minisequencing, A.C. Syvanen Oligonucleotide Ligation Assay, E.S. Winn-Deen Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis, A.J. Wallace Denaturing Gradient Gel Electrophoresis (DGGE), M. Loosekoot and R. Fodde Coupled Transcription-Translation, T. Hamzehleoi and S. West Peptide Nucleic Acids, H. Orum, T Koch, M. Egholm, H. O'Keefe, and J. Coull Orphan Peak Analysis, M. Hattori, H. Hagiwara, and Y. Sakaki Sequence-Specific Gene Signatures Produced by LSSP-PCR, A.J.G. Simpson and S.D.J. Pena Bisulphite Genomic Sequencing of Methylated Cytosines, S.J. Clark and M. Frommer Direct Sequencing of PCR Products, E. Werle Heterozygote Determination Using Automated DNA Sequencing Technology, M.N. Kronick A Robust Sequencing Protocol for PCR Products, M.D. Robinson and K.J. Leach Use of MutY and Thymine Glycosylase to Detect Point Mutations, I.C. Hsu MutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes, V.H. Laitinen, G.R. Taylor, S. Hamilton and P. Flick Enzyme Mismatch Cleavage-Bacterial Resolvases, R.D. Mashal and J. Sklar Chemical Cleavage of Mismatch in Heteroduplexes, J. Prosser Comparison of Mutation Detection by Chemical Cleavage of Mismatch (CCM) and Enzyme Mismatch Cleavage (EMC), R. Youil, S.J. Ramus, and R.G.H. Cotton Analysis of DNA Sequence Using Biosensors, P. Nilsson, B. Persson, M. Uhlen, and P.A. Nygren Repeat Expansion Detection, C. Zander, C. Erickson Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. Schalling Comparative Genomic Hybridization, A Kallioniemi, R. Karhu, and O.-P. Kallioniemi Large-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE), I.N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. Humphries DNA Sequence Database Searches, Comparisons, and Primer Design, G. Williams Accessing Genome Data: The Human Genome Database, Internet, and WWW, G. Williams List of Suppliers Index
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