Books  Animal & General Biology  Microbiology 

Membrane Transport: A Practical Approach

Handbook / Manual

Series: Practical Approach Series

Edited By: SA Baldwin

320 pages, Illus, figs, tabs

Oxford University Press

Paperback | Jun 2000 | #117292 | ISBN: 0199637040
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £79.99 $103/€88 approx

About this book

Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.


Assay of membrane transport in cells and membrane vesicles; Reconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulum; The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins; Expression of foreign transport proteins in yeast; Baculovirus-mediated overexpression of transport proteins; The amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins; Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters; Detection and analysis of glucose transporters using photolabelling techniques; Computer prediction of transporter topology and structure; Two-dimensional crystallization of membrane proteins

Write a review

There are currently no reviews for this product. Be the first to review this product!

Bestsellers in this subject

Defensive Mutualism in Microbial Symbiosis

NHBS Price: £118.00 $152/€129 approx

Life's Engines

NHBS Price: £14.95 $19/€16 approx

Bacteria and Fungi from Fish and Other Aquatic Animals

NHBS Price: £145.00 $187/€159 approx

Algal Toxins

NHBS Price: £96.99 $125/€106 approx

Deep Life

NHBS Price: £27.95 $36/€31 approx

VAT: GB 407 4846 44
NHBS Ltd is registered in England and Wales: 1875194