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This practical manual represents a comprehensive, up-to-date compilation of useful chemical ecology techniques and references. Written from the viewpoint of the practitioner, this book and its companion volume on bioassays describe apparatus and methods, providing detailed discussions of the advantages and limitations of various techniques. Taken together, the volumes provide the information required to isolate and identify biologically active chemicals mediating inter- and intraspecific interactions between organisms from most of the major taxa. "Methods in Chemical Ecology: Chemical Methods" describes both macro- and microscale techniques, paying particular attention to the problems inherent in working with microscale samples.The book is arranged in a logical sequence, beginning with chapters on the initial extraction and purification of compounds, and progressing through methods used in identification of chemical structures, including both instrumental and microchemical methods. The book finishes with chapters on the separation of enantiomers, and the use of electrophysiological techniques. Coverage includes descriptions of both cutting-edge methods such as solid phase microextraction, and methods that have been in common use for a decade or more. With minimal use of technical jargon, this volume is designed as an indispensable reference manual for graduate students as well as experienced researchers. This volume will also serve as a valuable reference book for researchers in many related disciplines, including natural-products chemistry, ecology, botany/plant sciences, zoology, entomology, marine biology and ecology, and pharmacology.
