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About this book
Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers tried and tested protocols for a range of detection methods, from the labs of researchers in the field.
Contents
1. Single strand conformation polymorphism analysis; 2. Single-stranded conformation polymorphism and heteroduplex analysis; 3. Comprehensive mutation detection with denaturing gradient gel electrophoresis; 4. Cleavage using RNase to detect mutations; 5. Cleavage of mismatched bases using chemical reagents; 6. Mutation detection using T4 endonuclease VII; 7. Detection of mutations by hybridization with sequence-specific oligonucleotide probes; 8. DNA detection and sequence distinction through oligonucleotide ligation; 9. Detection of sequence variation using primer extension; 10. Selective amplification of specific alleles; 11. The protein truncation text (PTT); 12. Functional assay of the p53 tumour suppressor gene; 13. Advances in direct DNA sequencing for mutation scanning
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