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As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The "Nanoproteomics: Methods and Protocols" volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled "Methods in Molecular Biology", these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.
Convenient and authoritative, "Nanoproteomics: Methods and Protocols" offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
Part I: Preliminary Sample Preparation
1. MALDI-TOF Serum Profiling Using Semi-Automated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads
2. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling
Part II: Nanoscale Separation
3. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces
4. Three Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures
5. Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species
6. Nanoelectrospray-MSn of Native and Permethylated Glycans
7. N-linked Global Glycan Profiling by NanoLC Mass Spectrometry
8. Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein-Protein Interaction, and Protein Phosphorylation
9. Nano LC-MS/MS: A Robust Setup for Proteomic Analysis
10. Nanofluidic Devices for Rapid Continuous-Flow Bioseparation
11. Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM)
Part III: Nanomaterials: Nanostructured Surfaces
12. A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
13. Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry
14. Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry
15. Protein Nanoarrays for High Resolution Patterning of Bacteria on Gold Surfaces
Part IV: Nanomaterials: Nanoscale Tools for Proteomic Discovery
16. Engineered Multifunctional Nanotools for Biological Applications
17. The Selective Capture of Phosphopeptides by Zirconium Phosphonate-Modified Magnetic Nanoparticles
18. Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection
19. Generation of Anti-Infectome/Anti-Proteome Nanobodies
Part V: Nanotechnology: Techniques in Proteomics--Potential Application for Health & Disease
20. Isolation, Propagation, and Analysis of Biological Nanoparticles
21. Functionalized Soluble Nanopolymers for Phosphoproteome Analysis
22. Elucidating Structural Dynamics of Integral Membrane Proteins on Native Cell Surface by Hydroxyl Radical Footprinting and NanoLC-MS/MS
23. Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC) to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS
