About this book
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. "PCR Applications" examines the latest developments in this field. It is the third book in the series, building on the previous publications "PCR Protocols" and "PCR Strategies". The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters; and, illustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR. The entries provide information on: nomenclature, expression, sequence analysis, structure and function, electrophysiology, parmacology, and information retrieval.
Contents
Key Concepts for PCR: M. Innis and D. Gelfand, Optimization of PCR: Conversations between Michael and David. J.J. Sninsky, The Convergence of PCR, Computers, and the Human Genome Project: Past, Present and Future. R.D. Abramson, Thermostable DNA Polymerases: An Update. B.R. Bloom, Musings on Microbial Genomes. E.M. Beasley, R.M. Myers, D.R. Cox, and L.C. Lazzeroni, Statistical Refinement of Primer Design Parameters. G. Zangenberg, R. Saiki, Multiplex PCR: Optimization Guidelines. R. Wagner and A.D. Dean, The Use of Immobilized Mismatch Binding Protein for the Optimization of PCR Fidelity. M.A. Northrup, L.A. Christel, W.A. McMillan, K. Petersen, F. Pourhamadi, L. Western, S. Young, A New Generation of PCR Instruments and Nucleic Acid Concentration Systems. J.M. Kelley and J. Quackenbush, Sequencing PCR Products. T.W. Myers, Recent Advances in High-Temperature Reverse Transcription and PCR. S. Kaye, Viral Genotyping by a Quantitative Point Mutation Assay: Application to HIV-1 Drug Resistance. J.R. Hully, In Situ PCR. Quantitative PCR: D.B. Bubois, C.R. WalkerPeach, M.M. Winkler, and B.L. Pasloske, Standards for PCR Assays. C.T. Wittwer and M.G. Herrmann, Rapid Thermal Cycling and PCR Kinetics. A.L.Hayward, P.J. Oefner, D.B. Kainer, C.A. Hinojos, and P.A. Doris, Kinetics of Competitive Reverse Transcriptase-PCR. R. Higuchi and R. Watson, Kinetic PCR Analysis Using a CCD Camera and without Using Oligonucleotide Probes. S.-Y.P. Chang, Quantification of Telomerase Activity Using Telomeric Repeat Amplification Protocol. Gene Discovery: K. Giese, H. Xin, J.C. Stephans, and X. Duan, Differential Display. P.S. Nelson, Single-Cell cDNA Libraries. J. Snider, Whole Cell Assays. F. Mathieu-Daude, N. Benson, F. Kullmann, R. Honeycutt, M. McClelland, and J. Welsh, Screening Differentially Displayed PCR Products by Single-Strand Conformation Polymorphism Gels. Y. Oh and L. Mao, Microsatellite Protocols. P.M. Williams and A.L. Tucker, Real-Time Quantitative PCR: Uses in Discovery Research. A.-L. Reysenbach and C. Vetriani, Homology Cloning: A Molecular Taxonomy of the Archaea. F. Randazzo, Cloning Mammalian Homologs of Drosophila Genes. T.Seeley, Cloning Human Homologs of Yeast Genes. Genomics and Expression Profiling: J.J. Kang and M.J. Holland, Cellular Transcriptome Analysis Using a Kinetic PCR Assay. M. Schena and R.W. Davis, Parallel Analysis with Biological Chips. A.O. Schmitt, R. Herwig, S. Meier-Ewert, and H. Lehrach, High Density cDNA Grids for Hybridization Fingerprinting Experiments. K. Chin and J.W. Gray, Comparative Genomic Hybridization. T. Ferea, B. Dunn, D. Botstein, and P. Brown, Genetic Footprinting and Fucntional Maps of the Yeast Genome. N.L. Simone, J.Y. Lee, M. Huckabee, K.A. Cole, R.F. Chaqui, C. Seshandri, B. Bonner, L.A. Liotta, and M.R. Emmert-Buck, Molecular Analysis of Microdissected Tissue: Laser Capture Microdissection. T. Pastinen, A.-C. Syvanen, C. Moberg, G. Sitbon, and J. Lonngren, A Fluorescent, Multiplex Solid-Phase Mini Sequencing Method for Genotyping Cytochrome P450 Genes. M.A.D. Brow, The Cleavase Enzyme for Mutation and Polymorphism Scanning. Index.
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