To see accurate pricing, please choose your delivery country.
 
 
United States
£ GBP
All Shops

British Wildlife

8 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £33 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £26 per year
Academic & Professional Books  Reference  Physical Sciences  Chemistry

PCR Protocols Current Methods and Applications

Handbook / Manual Out of Print
Series: Methods in Molecular Biology Volume: 15
Edited By: BA White
312 pages, Illus
Publisher: Humana Press
PCR Protocols
Click to have a closer look
  • PCR Protocols ISBN: 9780896032446 Paperback Dec 1993 Out of Print #26732
About this book Contents Related titles

About this book

Offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. The book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers.

Contents

Polymerase Chain Reaction. Selection of Primers for Polymerase Chain Reaction. Direct Radioactive Labeling of Polymerase Chain Reaction Products. Use of Arithmetic Polymerase Chain Reaction for Synthesis of Single-Stranded Probes for S1 Nuclease Assays. Nonradioactive Labeling of Polymerase Chain Reaction Products. Quantitation and Purification of Polymerase Chain Reaction Products by High-Performance Liquid Chromatography. Use of Polymerase Chain Reaction for Screening Transgenic Mice. Polymerase Chain Reaction Analysis of DNA from Paraffin-Embedded Tissue. The Use of Polymerase Chain Reaction for Chromosome Assignment. Mapping MHC Class II Genes and Disease-Susceptibility. The Use of the Polymerase Chain Reaction and the Detection of Amplified Products. Determination of Loss of Heterozygosity Using Polymerase Chain Reaction. Direct Sequencing of Polymerase Chain Reaction Products. Manual and Automated Direct Sequencing of Product Generated by the Polymerase Chain Reaction. Genomic Footprinting by Ligation Mediated Polymerase Chain Reaction. RNA Template- Specific Polymerase Chain Reaction (RS-PCR). Quantitative Measurement of Relative Gene Expression in Human Tumors. Identification of Alternatively Spliced mRNAs and Localization of 5' Ends by Polymerase Chain Reaction Amplification. Utilization of Polymerase Chain Reaction for Clonal Analysis of Gene Expression. Sequencing DNA Amplified Directly from a Bacterial Colony. Use of Polymerase Chain Reaction to Screen Phage Libraries. Molecular Cloning of Polymerase Chain Reaction Fragments with Cohesive Ends. Rapid (Ligase-Free) Subcloning of Polymerase Chain Reaction Products. Use of Polymerase Chain Reaction for Making Recombinant Constructs. In Vitro Recombination and Mutagenesis of DNA. Use of Polymerase Chain Reaction for the Rapid Construction of Synthetic Genes. Recombinant Circle Polymerase Chain Reaction for Site-Directed Mutagenesis. Site-Directed Mutagenesis by Double Polymerase Chain Reaction. Generation of a Polymerase Chain Reaction Renewable Source of Subtractive cDNA. PCR-Based Full-Length cDNA Cloning Utilizing the Universal-Adaptor/Specific DOS Primer-Pair Strategy. Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members. Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. cDNA Cloning by Inverse Polymerase Chain Reaction. Amplification of Gene Ends from Gene Libraries by Polymerase Chain Reaction with Single-Sided Specificity. Anchoring a Defined Sequence to the 5' Ends of mRNAs. Index.

Customer Reviews

Handbook / Manual Out of Print
Series: Methods in Molecular Biology Volume: 15
Edited By: BA White
312 pages, Illus
Publisher: Humana Press
Media reviews
...affords many new insights and contributions of the first order...an excellent book of the highest interest. - Cellular and Molecular Biology
Current promotions
New and Forthcoming BooksNHBS Moth TrapBritish Wildlife MagazineBuyers Guides