A comprehenive and cutting-edge collection of basic and advanced biochemical techniques for isolating, treating, and analyzing the photosynthetic materials and processes. All protocols follow the successful "Methods in Molecular Biology" series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
From the reviews: "This book series is well known as a competent tool for research protocols in all fields of life science. ! The book addresses either beginners in photosynthesis research or experienced researchers who want to extend their methodological experience. ! I can recommend the book as basic literature for advanced courses in photosynthesis. ! In conclusion, this is an excellent book which can be recommended for all laboratories involved in photosynthesis education and research." (Christian Wilhelm, Journal of Plant Physiology, Vol. 162 (6), 2005)
Photosynthesis Research Protocols; Edited by Robert Carpentier; Contents; 1 Fractionation of thylakoid membranes into grana and stroma thylakoids Juan Cuello and Maria Jose Quiles; 2 Isolation of photosystem I particles from spinach Tetsuo Hiyama; 3 Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii Velupillai M. Ramesh and Andrew N. Webber; 4 Isolation of photosystem II-enriched membranes and the oxygen-evolving complex subunit proteins from higher plants Yasusi Yamamoto, Shinsuke Sakuma, and Jian-Ren Shen; 5 Isolation of functional photosystem II core particles from the cyanobacterium Synechocystis sp. PCC 6803 Dmitrii Vavilin; 6 Isolation of photosystem I reaction center preparation from spinach Tetsuo Hiyama; 7 Isolation of photosystem II reaction center complexes from plants. Michael Seibert, Inmaculada Yruela, and Rafael Picorel; 8 Isolation of photosystem I reaction center subunit polypeptides from spinach Tetsuo Hiyama; 9 Purification of the cytochrome b6f complex in oxygenic photosynthesis Huamin Zhang and William A. Cramer; 10 Purification of plastocyanin and cytochrome C6 from plants, green algae, and cyanobacteria Jose A. Navarro, Manuel Hervas, and Miguel A. De la Rosa; 11 Preperation of native and recombinant light-harvesting chlorophyll-a/b complex Wolfgang Ruhle and Harald Paulsen; 12 Isolation and characterization of lamellar aggregates of LHCII and LHCII-lipid macro-assemblies with light-inducible structural transitions Ilian Simidjiev, Zsuzsanna Varkonyi, and Gyozo Garab; 13 Separation, purification and characterization of polypeptide composition of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II s Grzegorz Jackowski; 14 Isolation of CP43 and CP47 photosystem II proximal antenna complexes for plants Rafael Picorel, Miguel Alfonso, and Michael Seibert; 15 The determination and quantitication of photosynthetic pigments by reverse phase high performance liquid chromatography (HPLC), thin layer chromatography, and spectroscopy Tessa Pocock, Marianna Krol, and Norman P. A. Huner; 16 Isolation and identification of chloroplast lipids Norihiro Sato and Mikio Tsuzuki; 17 DNA adducts with chlorophyll and chlorophyllin as antimutagenic agents. Synthesis, stability, and structural features Heidar-Ali Tajmir-Riahi, Jean-Francois Neault, and Stravoula Diamantoglou; 18 Incorporation and analysis of LHCII in model systems Wieslaw I. Gruszecki; 19 Photosystem II reconstitution into proteoliposomes: Structure-function characterization Mario Fragata; 20 Extraction of the functional manganese and calcium from photosystem II Joel Freeman, Garth Hendry, and Tom Wydrzynski; 21 Assay of photoinhibition of photosystem II and protease activity Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, and Satoshi Ohira; 22 Thermoluminescence: A technique for probing photosystem II Prafullachandra V. Sane; 23 Detection of free radicals and reactive o
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