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Plant Cell Culture: Essential Methods

Handbook / ManualCancelled

Edited By: Michael R Davey and Paul Anthony

341 pages, Figs, tabs

Scion Publishing Ltd

Hardback | Nov 2009 | #181087 | ISBN-13: 9781904842514
Publication cancelled Details

About this book

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to "Plant Cell Culture: Methods Express" will provide a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter will provide background to the topic area and methodology, a crucial aspect will be the provision of detailed protocols with emphasis on troubleshooting, describing common problems and detailed advice for their avoidance.

"Plant Cell Culture: Methods Express" provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.


1. Introduction Michael Davey and Paul Anthony, both at the University of Nottingham 2. Plant micropropagation Ivan Iliev, University of Forestry, Sofia, Bulgaria; Alena Gajdosova and Gabriela Libiakova, both at the Institute of Plant Genetics and Biotechnology SAS, Slovakia; and S. Mohan Jain, Department of Applied Biology of the University of Helsinki, PL-27, Helsinki, Finland 3. Cistanche deserticola cell suspension cultures for phenylethanoid glycosides production Xi-Yu Cheng and Chun-Zhao Liu, both at the National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing; and Praveen K. Saxena, Department of Plant Agriculture, University of Guelph, Canada 4. Thin cell layers: the technique J. Texiera da Silva, Kagawa University 5. Plant regeneration - somatic embryogenesis Kim Nolan and R. Rose, both at the University of Newcastle, Australia 6. Haploid plants S. Bhojwani, Agra, India 7. Embryo rescue Traud Winkelman, University of Applied Sciences Weihenstephan, Germany 8. Induced mutagenesis in plants using physical and chemical agents Chikelu Mba, Rownak Afza, and Souleymane Bado, all at the Plant Breeding Unit, Joint FAO/IAEA Agriculture and Biotechnology Laboratory, International Atomic Energy Agency Laboratories, Austria; and S. Mohan Jain, Helsinki University 9. Cryopreservation of plant germplasms Joachim Keller and Angelika Senula, both at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) 10. Protoplasts - isolation, culture and plant regeneration Michael Davey, University of Nottingham 11. Protoplast fusion technology - somatic hybridisation and cybridisation J. Grosser and Milica A alovia, both at the University of Florida; and Eliezer S. Louzada, Texas A&M University 12. Genetic transformation - Agrobacterium I. Curtis, Agrilife Research Center - Weslaco 13. Genetic transformation - biolistics Fredy Altpeter and Sukhpreet Sandhu, both at the University of Florida 14. Plastid transformation Cilia L.C. Lelivelt, Rijk Zwaan Breeding B.V., The Netherlands; Bridget V. Hogg, Aisling Dunne, Kim-Hong Nguyen and Jacqueline Nugent, all at the Institute of Bioengineering and Agroecology, National University of Ireland 15. Molecular characterisation of genetically manipulated plants E. Rech, EMBRAPA 16. Bioreactors S. Kintzios, Agricultural University of Athens 17. Secondary products Ke X. Tang, Lei Zhang, Jun F. Chen, Ying Xiao and Wan S. Chen, Shanghai Jiao Tong University 18. Somatic cell technologies: progress, prospects and plant improvement J.M. Dunwell, University of Reading List of suppliers Index

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