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About this book
Contents
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About this book
Comprehensive guide to background theory and experimental methods.
Contents
Plant Genome Constituents and Their Organization; LIBRARY CONSTRUCTION: cDNA Library Construction; Genomic Library Construction; BAC, YAC and Cosmid Library Construction; LIBRARY SCREENING: Heterologous and Homologous Gene Probes; Differential Screening; Expression Library Screening; Functional Complementation in Yeast and E. coli; MAP-BASED CLONING: Classical Mutagenesis and Gene Mapping; Chromosome Walking; INSERTIONAL MUTAGENESIS: T-DNA Mediated Insertional Mutagenesis; Transposon Mediated Insertional Mutagenesis; PCR-BASED CLONING: PCR Techniques; cDNA and Genomic Subtraction; SEQUENCING PROJECTS: EST and Genomic Sequencing Projects.
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