Designed for use in the classroom, this edition contains both fundamental and recently described techniques in the area of plant molecular biology. it presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis.
Supplement II: RNA Differential Display; M.J. Laskowski. Expression Analysis of Multigene Families by RFLP-Coupled Domain-Directed Differential Display (RC4D); A. Fischer, G. Theissen, H. Saedler. Recombinant Aequorin Methods for Measurement of Intracellular Calcium in Plants; H. Knight, A.J. Trewavas, M.R. Knight. GUS Activity Staining - A Powerful Tool in Plant Molecular Biology; Jeong Hee Lee, F. Schoffl. In-Cell RT-PCR in a Single, Detached Plant Cell; Ho-Hyung Woo.
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