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Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools.
In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression.
Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.
Preface Contributors
1. Role of Model Plant Species
2. New Technologies for Ultra-High Throughput Genotyping in Plants
3. Genetic Maps and the Use of Synteny
4. A Simple TAE-based Method to Generate Large Insert BAC Libraries from Plant Species
5. Transcript Profiling and Expression Level Mapping
6. Methods for Functional Proteomic Analyses
7. Stable Transformation of Plants
8. Transient Transformation of Plants
9. Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics
10. Heterologous and Cell Free Protein Expression Systems
11. Functional Genomics and Structural Biology in the Definition of Gene Function
12. In situ Analysis of Gene Expression in Plants
13. Plant and Crop Databases
14. Plant Genome Annotation Methods
15. Molecular Plant Breeding: Methodology and Achievements
16. Practical Delivery of Genes to the Marketplace
17. Ecological Genomics of Natural Plant Populations: The Israeli Perspective
18. Genome Sequencing Approaches and Successes
From the reviews: "The book is latest volume in the series 'Methods in Molecular Biology' ! . The volume 'Plant Genomics. Methods and Protocols' concerns a wide spectrum of problems, some of them outside of the main scope of the field ! . In summary, 'Plant Genomics. Methods and Protocols' can be recommended as a valuable source of timely information for students, researchers and breeders." (PaweAC SowiA ski, Acta Biochimica Polonica, December, 2009) "This book is part of the very successful Methods in Molecular Biology series that provides research protocols and methodologies for biological scientists. ! the book does succeed in covering a wide range of topics and will therefore be a useful addition to university libraries. ! In summary, the book is well written with some informative and detailed protocols for plant molecular biologists ! ." (Stephen Byrne and Toni Wendt, Annals of Botany, Vol. 107 (4), 2011)
