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Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity.
Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.
Part I. Microelectrode Techniques
1. Patch-Clamp Protocols to Study Cell Ionic Homeostasis under Saline Conditions Ana Maria Velarde-Buendia, Rene Alberto Enriquez-Figueroa, and Igor Pottosin
2. Studying Plant Salt Tolerance with the Voltage Clamp Technique Zhong-Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, and Michael R. Blatt
3. Using the Multifunctional Xylem Pprobe for In situ Studies of Plant Water and Ion Relations under Saline Conditions Lars H. Wegner
4. Measuring Intracellular Ion Concentrations with Multi-Barrelled Microelectrodes Tony Miller and Susan Smith
5. Single-Cell Sampling and Analysis (SiCSA) Wieland Fricke
6. Non-Invasive Flux Measurements Using Microsensors: Theory, Limitations and Systems Ian Newman, Shao-Liang Chen, D. Marshall Porterfield, and Jian Sun
7. Quantifying Kinetics of Net Ion Fluxes from Plant Tissues by Non-Invasive Microelectrode Measuring MIFE Technique Sergey Shabala, Tracey Cuin, Lana Shabala and Ian Newman
Part II. Imaging Techniques
8. Quantitative Cryo-Analytical Scanning Electron Microscopy (CEDX): An Important Technique Useful for Cell-Specific Localization of Salt Margaret McCully and Martin Canny
9. Fluorescence Lifetime Imaging (FLIM) Measurements in Salinity Research Olga Babourina and Zed Rengel
10. Cytosolic Ca2+ Determinations in Studying Plant Responses to Salinity and Oxidative Stress Anuphon Laohavisit, Renato Colaco, and Julia Davies
11. Infrared Thermography in Plant Phenotyping for Salinity Tolerance Richard A. James and Xavier R.R. Sirault
12. In vivo Imaging of Nitric Oxide and Reactive Oxygen Species Using Laser Scanning Confocal Microscopy Yan-Jie Xie and Wen-Biao Shen
Part III. Biochemical Assays
13. Metabolomics for Salinity Research Ute Roessner and Diane M. Beckles
14. Purification of Plant Plasma Membranes by Two-Phase Partitioning and Measurement of H+ Pumping Anette Lund and Anja Thoe Fuglsang
15. Determination of Reactive Oxygen Species in Salt-Stressed Plant Tissues Andres Alberto Rodriguez and Edith L. Taleisnik
16. Quantification of the Antioxidant Activity in Salt-Stressed Tissues Jelena J. Dragisic Maksimovic and Branka D. Zivanovic
17. Quantification of Abscisic Acid, Cytokinin and Auxin Content in Salt-Stressed Plant Tissues Petre I. Dobrev and Radomira Vankova Part IV. Molecular Techniques
18. Fluorescence-Activated Cell Sorting for Analysis of Cell Type-Sspecific Responses to Salinity Stress in Arabidopsis and Rice Aurelie Evrard, Bastiaan O. R. Bargmann, Kenneth D. Birnbaum, Mark Tester, Ute Baumann, and Alexander A. T. Johnson
19. Transformation Using Controlled cDNA Overexpression System Gabor Rigo, Csaba Papdi, and Laszlo Szabados
20. Transcriptome Analysis of Membrane Transporters in Response to Salinity Stress Prasad Senadheera and Frans J.M. Maathuis
21. Marker-Assisted Selection in Plant Breeding for Salinity Tolerance M. Ashraf, N. A. Akram, Mehboob-ur-Rahman, and M. R. Foolad
22. Transcriptomics on Small Samples Stuart J. Roy, Simon J. Conn, Gwenda M. Mayo, Asmini Athman, and Matthew Gilliham
23. Plastid Transformation for Abiotic Stress Tolerance in Plants K.C. Bansal, A. K. Singh, and S. H. Wani
24. Manipulating Expression of Tonoplast Transporters Zhigang Li, Man Zhou, Qian Hu, Shane Reighard, Shuangrong Yuan, Ning Yuan, Bekir San, Dayong Li, Haiyan Jia, and Hong Luo
25. Using Heterologous Expression Systems to Characterize Potassium and Sodium Transport Activities Alonso Rodriguez-Navarro1, Begona Benito, and Olivier Cagnac Part V. Other Methods
26. Isotope Techniques to Study Kinetics of Na+ and K+ Transport Under Salinity Conditions D.T. Britto and H.J. Kronzucker
27. Trait Dissection of Salinity Tolerance with Plant Phenomics Bettina Berger, Bas de Regt, and Mark Tester
28. Measuring Soil Salinity Marcus Hardie and Richard Doyle
