Polyamine Protocols

About this book

A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Contents

Part I. Introduction. Polyamines: An Introduction D. Morgan. Part II. Assay Methods for Enzymes of Polyamine Biosynthesis. Determination of Ornithine Decarboxylase Activity Using [3H]Ornithine A. Tabib. Assay of Mammalian Ornithine Decarboxylase Activity Using [14C]Ornithine C. Coleman and A. Pegg. Assay of Mammalian S-Adenosylmethionine Decarboxylase Activity L. Shantz and A. Pegg. Assay of Spermidine and Spermine Synthases L. Wiest and A. Pegg. Measurement of Spermidine/Spermine N1-Acetyltransferase Activity H. Wallace and D. Evans. Assay of Spermidine N8-Acetyltransferase J. Blankenship. Part III. Assay Methods for Enzymes of Polyamine Catabolism. Assay of N8-Acetylspermidine Deacetylase J. Blankenship. Hydrogen Peroxide Assay for Amine Oxidase Activity R. Storer and A. Ferrante. Radiochemical Assay of Diamine Oxidase R. Storer and A. Ferrante. Radiochemical Estimation of Polyamine Oxidase D. Morgan. Measurement of Aldehyde Formation as an Assay for Polyamine Oxidase Activity D. Morgan. Part IV. Measurement of Polyamines. Determination of Polyamines as Their Benzoylated Derivatives by HPLC D. Morgan. A Dansyl Chloride-HPLC Method for the Determination of Polyamines K. Hunter. The Determination of Polyamines and Amino Acids by a Fluorescamine- HPLC Method K. Hunter and A. Fairlamb. Thin-Layer Chromatographic Method for Assaying Polyamines R.Madhubala. Part V. Measurement of Polyamine Transport. Measurement of Polyamine Transport: Adherent Cells D. Morgan. Measurement of Polyamine Transport: Cells in Suspension S. Le Quesne and A. Fairlamb. Measurement of Polyamine Efflux from Cells in Culture H. Wallace and A. Mackarel. Part VI. Measurement of Polyamine Effects on Cell Growth. Leucine Incorporation and Thymidine Incorporation C. Denton. Tetrazolium (MTT) Assay for Cellular Viability and Activity D. Morgan. Index.

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