Protein Phosphate Protocols

About this book

Cutting-edge techniques presented with foolproof, step-by-step instructions make this collection indispensable for investigating the structure and function of protein serine/threonine phosphatases. The book contains methods designed to enable the investigator to isolate, identify, and assay phosphatase activities from both prokaryotic and eukaryotic sources, and includes a discussion of the functional significance of this group of enzymes with respect to cell growth and development. Written by leaders with extensive practice in their use, each method is time-tested and sufficiently detailed to ensure reproducibility for both new and established investigators.

Contents

Prokaryotic Protein-Serine/Threonine Phosphatases P. J. Kennelly. Protein Phosphatase Type 1 and 2A Assays S. D. Killilea Q.Cheng and Z. X. Wang. Analyzing Gene Expression with the Use of Serine/Threonine Phosphatase Inhibitors A. H. Shnthal. Inhibitor-1 a Regulator of Protein Phosphatase-1 Function J. H. Connor H. Q. C. Oliver and S. Shenolikar. I1PP2A and I2PP2A: Two Potent Protein Phosphatase 2A-Specific Inhibitor Proteins M. L. and Z. Damuni. Control of PP1 Activity Through Phosphorylation by Cyclin-Dependent Kinases N. Berndt. Regulation of Neuronal PP1 and PP2A During Development E. Collins and A.T. R. Sim. PTPA Regulating PP2A as a Dual Specificity Phosphatase V.Janssens C. Van Hoof W. Merlevede and J. Goris. Microinjection and Immunological Methods in the Analysis of Type 1 and Type 2A Protein Phosphatases from Mammalian Cells P.Turowski and N. J. C. Lamb. Use of Immunocomplexed Substrate for Detecting PP1 Activity J. W. Ludlow D. A. Nelson and N. A. Krucher. The Biochemical Identification and Characterization of New Species of Protein Phosphatase 1 M. Beullens W. Stalmans and M. Bollen. The Relationship between insulin signaling and protein phosphatase 1 activation L. Ragolia and N. Begum. Analysis of the isoforms of protein phosphatase (PP1) isoforms with polyclonal peptide antibodies M. Tognarini and E. Villa-Moruzzi. Expression of mouse protein phosphatase 2C in Eschericia coli and COS 7 cells Takayasu Kobayashi Kazuyuki Kusuda Motoko Ohnishi Naoki Chida and Shinri Tamura. Expression of Functional protein phosphatase 1 catalytic subunit in Eschericia coli M. Dohadwala and N. Berndt . Protein Phosphatase 2A and Protein Phosphatase X Genes in Arabidopsis thaliana G. Pujol A. Ferrer and J. Ari¤o. Separation of Protein Phosphatase Type 2C Isozymes by Chromatography on Blue Sepharose S. Klumpp and D. Selke. Chromatographic Isolation of PP2A from Limulus Lateral Eyes: Conventional and Small Scale Methods S. C. Edwards T. B. Van Dyke T. H. Van Dyke and D. L. Brautigan. Purification and Assay of the Ptc/Tpd1 Protein Phosphatase 2C from the yeast Saccharomyces cerevisiae M. K. Robinson and E. M. Phizicky. Molecular cloning of protein phosphatase type 2C isoforms from retinal cDNA Da. Selke S. Klumpp B. Kaupp and A. Baumann. Analysis of Protein Interactions Between Protein Phosphatase 1 and Noncatalytic Subunits using the Yeast Two-Hybrid Assay N. T. Ramaswamy B. K. Dalley and J. F. Cannon. Identifying Protein Phosphatase 2A Interacting Proteins Using the Yeast Two-hybrid method B.McCright and D.M. Virshup. Protein phosphatase 2A regulatory subunits: cDNA cloning and analysis of mRNA expression J. A. Zaucha R. S. Westphal and B. E. Wadzinski. Synthetic Lethal Screening in Protein Phosphatase Pathways J. Zheng and J. F. Cannon. The Search for the Biological Function of Novel Yeast ser/thr Phosphatases J. Ari¤o F. Posas and J. Clotet. Index.

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