Protein Arrays Methods and Protocols

About this book

A diverse collection of unique methodologies to synthesize and construct protein arrays for basic and clinical research in a high-throughput manner. These readily reproducible techniques can be applied to such applications as quantifying specific proteins of interest and discovering novel proteins, as well as to a variety of affinity substances, including antibodies, peptides, proteins, aptamers, and chemicals. The techniques can be used for biomarkers discovery, assay development, clinical sample testing, and building the next generation of molecular tools.

Contents

Table of Contents; Protein Arrays: Methods and Protocols Edited by Eric T. Fung; 1 Protein Arrays from cDNA Expression Libraries Konrad Bussow, Hendrik Weiner, and Thomas Faupel; 2 Protein Expression Arrays for Proteomics Joanna Albala, Michele Gilbert, and Todd C. Edwards; 3 Generation of Protein In Situ Arrays by DiscernArray[trademark] Technology Mingyue He; 4 Multiplexed protein analysis using spotted antibody microarrays Brian B. Haab and Heping Zhou; 5 Target Assisted Iterative Screening of Phage Surface Display cDNA Libraries Alexei Kurakin, Susan Wu, and Dale E. Bredesen; 6 Design, Construction, and Use of Tissue Microarrays Stephen M. Hewitt; 7 A Streptavidin-Biotin Based Microarray Platform for Immunoassays Petra Pavlickova and Hubert Hug; 8 Site-Specific Immobilization of Biotinylated Proteins for Protein Microarray Analysis Rina Y. P. Lue, Grace Y. J. Chen, Qing Zhu, Marie-Laure Lesaicherre, and Shao Q. Yao; 9 Processing of Photoaptamer Microarrays Helen Petach, Rachel Ostroff, Chad Greef, and Greg Husar; 10 Use of a Small Molecule-Based Affinity System for the Preparation of Protein Microarrays Karin A. Hughes; 11 Evaluation of Antibodies and Microarray Coatings as a Prerequisite for the Generation of Optimised Antibody Microarrays Phillipp Angenendt and Jorn Glokler; 12 Printing Functional Protein Microarrays Using Piezoelectric Capillaries James B. Delehanty; 13 The Use of Precision Glass Syringes and a Non-Contact Microsolenoid Dispenser for the Production of High Throughout Low Density Arrays Arezou Azarani; 14 A Protein Microarray ELISA for Screening Biological Fluids Susan M. Varnum, Ronald L. Woodbury, and Richard C. Zangar; 15 Protein-Domain Microarrays Alexsandra Espejo and Mark T. Bedford; 16 SH3 Domain Protein Binding Arrays Sangpen Chamnongpol and Xianqiang Li; 17 Site-Specific Peptide Immobilization Strategies for the Rapid Detection of Kinase Activity on Microarrays Mahesh Uttamchandani, Grace Y. J. Chen, Marie-Laure Lesaicherre, Shao Q. Yao; 18 Simultaneous Monitoring of Multiple Kinases Activities by SELDI-TOF Mass Spectrometry Vanitha Thulasiraman, Zheng Wang, Anjali Katrekar, Lee Lomas, and Tai-Tung Yip; 19 Cytokine Antibody Arrays Ruo-Pan Huang; 20 Fabrication and Application of G Protein-Coupled Receptor Microarrays Ye Fang, Brian Webb, Yulong Hong, Ann Ferrie, Fang Lai, Anthony G. Frutos, and Joydeep Lahiri; 21 ProteinChip[registered] Array-Based Amyloid Beta Assays Lisa E. Bradbury, James F. LeBlanc, and Diane B. McCarthy; 22 Serum Protein Expression Profiling Using the ProteinChip[registered] Biomarker System Kate Gilbert, Sharel Figueredo, Xiao-Ying Meng, Christine Yip, and Eric T. Fung; 23 Protein Arrays for Serodiagnosis of Disease Tito Bacarese-Hamilton, Andrea Ardizzoni, Julian Gray, and Andrea Crisanti

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