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Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty.
Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.
1. How It All Began -- A Personal History of Gel-Electrophoresis Oliver Smithies
2. Introduction to Protein Electrophoresis Pothur R. Srinivas
3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis J.P. Dean Goldring
4. Protein Solubilization: Attend to the Choice of Lysis Buffer Mandy Peach, Noelle Marsh, and Daniel J. MacPhee
5. Native Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann
6. Cationic Electrophoresis Engelbert Buxbaum
7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins Christian Reichel
8. Tricine-SDS-PAGE Syed R. Haider, Helen J. Reid, and Barry L. Sharp
9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen
10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins Marion L. Greaser and Chad M. Warren
11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis Henghang Tsai and Hon-chiu Eastwood Leung
12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann
13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies Gyorgy Csako
14. Microchip Electrophoresis, with respect to "Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy
15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols Mustafa Celebier, Clara Ibanez, Carolina Simo, and Alejandro Cifuentes
16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra
17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa
18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting Edward P. Trieu and Ira N. Targoff
19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing Yaser Dorri
20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids Gyorgy Csako
21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution Biji T. Kurien and R. Hal Scofield
22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori
23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta
24. Two-Dimensional Difference Gel Electrophoresis Jonathan S. Minden
25. Protein Identification on Archived 2-D Gels Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori
26. Diagonal Electrophoresis for the Detection of Protein Disulfides Brian McDonagh
27. Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes Jean Paul Lasserre and Armelle Menard
28. Immunoelectrophoresis: A Method with Many Faces Gyorgy Csako
29. Cell Surface Protein Biotinylation for SDS PAGE Analysis Giuliano Elia
30. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin
31. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome Xianchun Wang and Songping Liang
32. Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test Yan Su, Lijun Gao, Wenbin Qin
33. Extraction of Proteins from Gels - A Brief Review Biji T. Kurien and R. Hal Scofield
34. A New Algorithm for the Simulation of SDS 2D-PAGE Datasets Emilio Marengo and Elisa Robotti
35. Isolation of Proteins from Polyacrylamide Gels Irene Michalk, Stefanie Koristka, Claudia Arndt, and Michael Bachmann
36. Gel Drying Methods Slava Stamova, Irene Michalk, Holger Bartsch, and Michael Bachmann
37. Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags Biji T. Kurien and R. Hal Scofield
38. A Brief Review of Other Notable Electrophoretic Methods James D. Fesmire
39. Protein Stains and Applications Ranjini K. Sundaram, Natarajan Balasubramaniyan, and Pazhani Sundaram
40. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels Claudia Arndt, Stefanie Koristka, Anja Feldmann, Holger Bartsch, and Michael Bachmann
41. Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat Biji T. Kurien and R. Hal Scofield
42. Silver Staining Techniques of Polyacrylamide Gels Holger Bartsch, Claudia Arndt, Stefanie Koristka, Marc Cartellieri, and Michael Bachmann
43. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis Han-Min Chen
44. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry Jung-Kap Choi
45. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE Na Na and Jin Ouyang
46. Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins Jennie R. Lill and Victor J. Nesatyy
47. Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping Tripti Khare, Yasmin Chishti, and Lydia A. Finney
48. Fluorescent Staining of Gels Engelbert Buxbaum
49. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni
50. Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain Padmaja Mehta-D'souza
51. Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain Biji T. Kurien, Yaser Dorri, and R. Hal Scofield
52. In-Gel Staining of Proteins in Native Poly Acryl Amide Gel Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin K. Divakar, G. Nandhini Devi, and Pennathur Gautam
53. Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black J.P. Dean Goldring, Robert G.E. Krause, and David Choveaux
54. Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels Mircea Alexandru Mateescu, Carmen Calinescu, Tien Canh Le, Rodolfo Federico, and Bruno Mondovi
55. Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner Biji T. Kurien and Yaser Dorri
56. A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels Biji T. Kurien and R. Hal Scofield
57. Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein(t), ProteoSilver(t), SYPRO(R) Ruby, and Coomassie(R) Brilliant Blue Gel Stains Guang Ren, Carl K. Okerberg, and Suresh T. Mathews
58. Common Artifacts and Mistakes Made in Electrophoresis Biji T. Kurien and R. Hal Scofield
