This volume provides a set of protocols for the analysis of cloned genes. A set of techniques is described for the basic manipulation and mutagenesis of the cloned sequences. The rest of the volume is organized into a collection of techniques appropriate to the analytical route to be investigated; these include genome structure, sequence variation, gene expression, and protein function. Finally, a number of methods are described to take the step from the first gene to those with which it interacts.
...recommended...A notes section that follows each methods section may be the most useful feature. These notes are like having the author right there explaining why certain things are done or not done, what variations may be tolerated or useful, what might go wrong, and how to remedy it. - Biopharm Manufacturing
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