Books  Data Analysis & Modelling  Microscopy & Spectroscopy 

Quantitative Fluorescence Microscopy

Out of Print

By: F Rost

250 pages, B/w photos, illus, tabs

Cambridge University Press

Hardback | Aug 1991 | #17737 | ISBN: 0521394228
Out of Print Details

About this book

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

This most recent book by F.W.D. Rost, one of the pioneers in the arena of quantitative fluorescence microscopy, has a more focused and organized content than other recent, multi-authored books on similar topics. Y-L Wang, Trends in Cell Biology "...will be of great help to anybody embarking on quantitative fluorescence. It provides the reader with often neglected considerations on techniques, optics, fluorochromes, as well as instrumentation--all packaged neatly in a well organized, readable, rather slender volume." Boris Albini, Immunological Investigations " important source of information in the field of fluorescence microscopy which is essential in view of the rapid development of confocal laser microscopy." Microscopial Society of Canada Bulletin


Preface; Abbreviations and symbols; 1. Principles of fluorescence microscopy; 2. Instrumentation for microfluorometry; 3. Microfluorometric technique; 4. Microfluorometry: errors, standardization and data processing; 5. Applications of microfluorometry; 6. Principles of microspectrofluorometry; 7. Instrumentation: microspectrofluorometers; 8. Microspectrofluorometric technique; 9. Microspectrofluorometry: errors, standardization and data processing; 10. Applications of microspectrofluorometry; 11. Photobleaching, photoactivation and quenching; 12. Time-resolved fluorescence, phosphorescence and polarization; 13. Quantitative enzyme studies; 14. Flow cytometry Hans J. Tanke; 15. Scanning, video intensification and image processing; 16. History of quantitative fluorescence microscopy; Appendices; References; Index.

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