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Rapid Freezing, Freeze Fracture and Deep Etching

Handbook / ManualOut of Print

Series: Techniques in Modern Biochemical Microscopy

Edited By: NJ Severs and DM Shotton

John Wiley & Sons

Hardback | Dec 1995 | #37603 | ISBN: 0471014338
Out of Print Details

About this book

Includes novel developments such as fracturing and shadowing under ultra-low temperature and ultra-high vacuum conditions, improvements in rapid freeze techniques and cytochemical methods for labelling specific cell components.


Partial table of contents:; An Introduction to Freeze Fracture and Deep Etching (D. Shotton & N. Severs).; A Guide to Equipment for Production of Freeze--Fracture Replicas (T. Newman).; Freeze--Fracture Artifacts: How to Recognize and Avoid Them (R. Abeysekera & A. Robards).; High Pressure Freezing (J. Kiss & L. Staehelin).; Time--Resolved Analysis of Rapid Events (G. Knoll).; Freeze--Fracture Cytochemistry: An Explanatory Survey of Methods (N. Severs).; Lipid Localization by Membrane Perturbation: A Cautionary Tale (N. Severs).; Membrane Lipids and Model Membrane Systems (M. Hope & W. Rodrigueza).; Visualization of Basement Membrane Molecules and Supramolecular Assemblies (P. Yurchenco & G. Ruben).; Index.

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