Recombinant DNA Principles and Methodologies

About this book

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. It features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies. "Recombinant DNA Principles and Methodologies" discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology, nucleic acid biochemistry, restriction enzymes, cloning strategies, gel electrophoresis, southern and northern blotting, preparation of probes, phage lambda biology, cosmids and genome analysis, cloned gene expression, polymerase chain reaction ,conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.

Contents

Why Cloning?, James J. Greene Biochemistry of Nucleic Acids, Paul S. Miller Enzymology of Recombinant DNA, Frank J. Castora Basic Escherichia coli Genetics Useful for Recombinant DNA Techniques, Darren D. Sledjeski and Susan Garges Formulation of Cloning Strategies, John B. Dame Isolation and Purification of Nucleic Acids, James J. Greene Gel Electrophoresis, Southern and Northern Analysis, James J. Greene and Frank J. Castora Hybridization Probes, Frank J. Castora and James J. Greene cDNA Cloning and the Construction of Recombinant DNA, Dante S. Zarlenga Molecular Cloning by Plasmid Vectors, Paulina Balbas and Francisco Bolivar Phage Lambda: Basic Biology and Use as a Vector, Susan Frackman and Michael Feiss Cosmid Cloning, John Collins and Werner Lindenmaier Multipurpose Cloning Vectors, August J. Sick, Joseph M. Fernandez, and Jay M. Short Mammalian Expression Vectors, Ricky R. Hirschhorn and Nava Sarver Approaches to the Expression of Foreign Genes, Allan R. Shatzman Screening for Specific Recombinant Clones, Lucia Irene Gonzalez-Villasenor and Mark M. Manak Principles and Applications of the Polymerase Chain Reaction, Garbriela S. Dveksler and Carl W. Dieffenbach DNA Sequencing, Maurice A. Kashdan, Phillip T. Moen, Jr.,Venigalla B. Rao, and Bachtoi S. Rao Site-Directed Mutagenesis, Alfred Johnson and Marvin Reitz

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