![Signalling by Inositides: A Practical Approach]( "Signalling by Inositides: A Practical Approach")
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About this book
Contents
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About this book
The metabolism and functions of inositol phosphates impinge on various branches of biochemistry, physiology, and molecular biology, and methodological information is in consequence scattered far and wide. This book unites a selection of the most fundamental and commonly used techniques from leading international signal transduction laboratories, and brings together many valuable protocols for purifying and assaying inositides and related compounds. A novel feature is a catalogue of non-commercial sources of synthetic inositide analogues.
Contents
1. Assaying inositol phospholipid turnover in plant cells; 2. Measurement of inositol phosphate turnover in intact cells and cell-free systems; 3. Mass assay of inositol and its use to assay inositol polyphosphates; 4. Phospholipase C-gamma 1; 5. Baculovirus-promoted expression, purification, and functional assay of G-protein regulated PLC-beta isoenzymes; 6. Purification and assay of phospholipase C-delta; 7. The purification and assay of inositol polyphosphate-binding proteins; 8. Mass assay of inositol 1,4,5-trisphosphate and phosphatidylinositol 4,5-bisphosphate; 9. Desalting inositol polyphosphates by dialysis; 10. The Ins(1,4,5)P3 receptor; 11. Purification and assay of inositol hexakisphosphate kinase and diphosphoinositol pentakisphosphate kinase; 12. Phosphoinositide 3-kinase
Customer Reviews
Handbook / Manual
Out of Print
Edited By: Stephen Shears
250 pages
"The graphs, diagrams, tables, and few half-tone illustrations are of good to excellent quality...The book is small and easy to handle and read...Complete list of suppliers at the end of the book....This small, well-written book should be useful for those who do not regularly work in this field. It contains clearly written and presented lab protocols that should be easy for anyone to follow and make work in their own laboratory."--Doody's Journal
