Species Diagnostics Protocols PCR and Other Nucleic Acid Methods

About this book

Enables investigators to rapidly and correctly identify species using molecular biology techniques.

Contents

Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA. Isolation and Purification of Insect DNA. Isolation and Purification of Vertebrate DNAs. Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies. Molecular Identification of Phytopathogenic Viruses. Improved PCR Methods for Identification of Phytopathogenic Viruses. PCR Detection of HIV. The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV. The Identification of Dengue Virus Using PCR. The Design and Application of Ribosomal RNA-Targeted Fluorescent Oligonucleotide Probes for the Identification of Endosymbionts in Protozoa. Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes. The Use of RAPD for Generating Specific DNA Probes for Microorganisms. ITS-RFLP Matching for Identification of Fungi. Specific PCR Primers for the Identification of Endomycorrhizal Fungi. Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi. The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi. Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe. Detection and Characterization of Leishmania Parasites by DNA- Based Methods: Southern Blotting and PCR. The Molecular Identification of Trypanosomes. Detection and Identification of the Four Malaria Parasite Species Infecting Humans by PCR Amplification. The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify Onchocerca volvulus. The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Complex. PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Anopheles gambiae Complex. Restriction Fragment Length Polymorphism (RFLP) Analyses: Distinguishing African and European Honey Bees. Differential Screening for the Isolation of Species-Specific Sequences in the Anopheles farauti Complex. RAPD- PCR with Parasitic Hymenoptera. The Use of Selective Enrichment for the Isolation of Species- Specific DNA Probes for Insects. Index.

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